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Origin DNA Melting-An Essential Process with Divergent Mechanisms.

Title: Origin DNA Melting-An Essential Process with Divergent Mechanisms.
Name(s): Martinez, Matthew P, author
Jones, John M, author
Bruck, Irina, author
Kaplan, Daniel L, author
Type of Resource: text
Genre: Journal Article
Date Issued: 2017-01-11
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Origin DNA melting is an essential process in the various domains of life. The replication fork helicase unwinds DNA ahead of the replication fork, providing single-stranded DNA templates for the replicative polymerases. The replication fork helicase is a ring shaped-assembly that unwinds DNA by a steric exclusion mechanism in most DNA replication systems. While one strand of DNA passes through the central channel of the helicase ring, the second DNA strand is excluded from the central channel. Thus, the origin, or initiation site for DNA replication, must melt during the initiation of DNA replication to allow for the helicase to surround a single-DNA strand. While this process is largely understood for bacteria and eukaryotic viruses, less is known about how origin DNA is melted at eukaryotic cellular origins. This review describes the current state of knowledge of how genomic DNA is melted at a replication origin in bacteria and eukaryotes. We propose that although the process of origin melting is essential for the various domains of life, the mechanism for origin melting may be quite different among the different DNA replication initiation systems.
Identifier: FSU_pmch_28085061 (IID), 10.3390/genes8010026 (DOI), PMC5295021 (PMCID), 28085061 (RID), 28085061 (EID), genes8010026 (PII)
Keywords: DNA helicase, DNA replication, DnaA, E1 helicase, Large T antigen, Mcm2–7, Initiation, Melting, Protein-DNA interaction
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at
Persistent Link to This Record:
Owner Institution: FSU
Is Part Of: Genes.
Issue: iss. 1, vol. 8

Choose the citation style.
Martinez, M. P., Jones, J. M., Bruck, I., & Kaplan, D. L. (2017). Origin DNA Melting-An Essential Process with Divergent Mechanisms. Genes. Retrieved from