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Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.

Title: Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
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Name(s): Hurwitz, Stephanie N, author
Rider, Mark A, author
Bundy, Joseph L, author
Liu, Xia, author
Singh, Rakesh K, author
Meckes, David G, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2016-12-27
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Packed with biological information, extracellular vesicles (EVs) offer exciting promise for biomarker discovery and applications in therapeutics and non-invasive diagnostics. Currently, our understanding of EV contents is confined by the limited cells from which vesicles have been characterized utilizing the same enrichment method. Using sixty cell lines from the National Cancer Institute (NCI-60), here we provide the largest proteomic profile of EVs in a single study, identifying 6,071 proteins with 213 common to all isolates. Proteins included established EV markers, and vesicular trafficking proteins such as Rab GTPases and tetraspanins. Differentially-expressed proteins offer potential for cancer diagnosis and prognosis. Network analysis of vesicle quantity and proteomes identified EV components associated with vesicle secretion, including CD81, CD63, syntenin-1, VAMP3, Rab GTPases, and integrins. Integration of vesicle proteomes with whole-cell molecular profiles revealed similarities, suggesting EVs provide a reliable reflection of their progenitor cell content, and are therefore excellent indicators of disease.
Identifier: FSU_pmch_27894104 (IID), 10.18632/oncotarget.13569 (DOI), PMC5341331 (PMCID), 27894104 (RID), 27894104 (EID), 13569 (PII)
Keywords: Biomarkers, Co-inertia, Exosomes, Microvesicles, Proteomics
Grant Number: R01 CA204621, R15 CA188941
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5341331.
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_27894104
Owner Institution: FSU
Is Part Of: Oncotarget.
1949-2553
Issue: iss. 52, vol. 7

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Hurwitz, S. N., Rider, M. A., Bundy, J. L., Liu, X., Singh, R. K., & Meckes, D. G. (2016). Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. Oncotarget. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_27894104