You are here

Intercellular Coupling of the Cell Cycle and Circadian Clock in Adult Stem Cell Culture.

Title: Intercellular Coupling of the Cell Cycle and Circadian Clock in Adult Stem Cell Culture.
4 views
0 downloads
Name(s): Matsu-Ura, Toru, author
Dovzhenok, Andrey, author
Aihara, Eitaro, author
Rood, Jill, author
Le, Hung, author
Ren, Yan, author
Rosselot, Andrew E, author
Zhang, Tongli, author
Lee, Choogon, author
Obrietan, Karl, author
Montrose, Marshall H, author
Lim, Sookkyung, author
Moore, Sean R, author
Hong, Christian I, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2016-12-01
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Circadian clock-gated cell division cycles are observed from cyanobacteria to mammals via intracellular molecular connections between these two oscillators. Here we demonstrate WNT-mediated intercellular coupling between the cell cycle and circadian clock in 3D murine intestinal organoids (enteroids). The circadian clock gates a population of cells with heterogeneous cell-cycle times that emerge as 12-hr synchronized cell division cycles. Remarkably, we observe reduced-amplitude oscillations of circadian rhythms in intestinal stem cells and progenitor cells, indicating an intercellular signal arising from differentiated cells governing circadian clock-dependent synchronized cell division cycles. Stochastic simulations and experimental validations reveal Paneth cell-secreted WNT as the key intercellular coupling component linking the circadian clock and cell cycle in enteroids.
Identifier: FSU_pmch_27867006 (IID), 10.1016/j.molcel.2016.10.015 (DOI), PMC5423461 (PMCID), 27867006 (RID), 27867006 (EID), S1097-2765(16)30638-4 (PII)
Keywords: Paneth cell, WNT signaling, Cell cycle, Circadian rhythm, Coupled oscillators, Intercellular coupling, Intestinal crypt, Intestinal stem cell, Progenitor cell
Grant Number: R01 DK102551, R01 NS091302, U19 AI116491
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5423461.
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_27867006
Owner Institution: FSU
Is Part Of: Molecular cell.
1097-4164
Issue: iss. 5, vol. 64

Choose the citation style.
Matsu-Ura, T., Dovzhenok, A., Aihara, E., Rood, J., Le, H., Ren, Y., … Hong, C. I. (2016). Intercellular Coupling of the Cell Cycle and Circadian Clock in Adult Stem Cell Culture. Molecular Cell. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_27867006