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Anti-Inflammatory Mechanism of Neural Stem Cell Transplantation in Spinal Cord Injury.

Title: Anti-Inflammatory Mechanism of Neural Stem Cell Transplantation in Spinal Cord Injury.
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Name(s): Cheng, Zhijian, author
Zhu, Wen, author
Cao, Kai, author
Wu, Fei, author
Li, Jin, author
Wang, Guoyu, author
Li, Haopen, author
Lu, Ming, author
Ren, Yi, author
He, Xijing, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2016-08-23
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Neural stem cell (NSC) transplantation has been proposed to promote functional recovery after spinal cord injury. However, a detailed understanding of the mechanisms of how NSCs exert their therapeutic plasticity is lacking. We transplanted mouse NSCs into the injured spinal cord seven days after SCI, and the Basso Mouse Scale (BMS) score was performed to assess locomotor function. The anti-inflammatory effects of NSC transplantation was analyzed by immunofluorescence staining of neutrophil and macrophages and the detection of mRNA levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6) and interleukin-12 (IL-12). Furthermore, bone marrow-derived macrophages (BMDMs) were co-cultured with NSCs and followed by analyzing the mRNA levels of inducible nitric oxide synthase (iNOS), TNF-α, IL-1β, IL-6 and IL-10 with quantitative real-time PCR. The production of TNF-α and IL-1β by BMDMs was examined using the enzyme-linked immunosorbent assay (ELISA). Transplanted NSCs had significantly increased BMS scores (p < 0.05). Histological results showed that the grafted NSCs migrated from the injection site toward the injured area. NSCs transplantation significantly reduced the number of neutrophils and iNOS+/Mac-2+ cells at the epicenter of the injured area (p < 0.05). Meanwhile, mRNA levels of TNF-α, IL-1β, IL-6 and IL-12 in the NSCs transplantation group were significantly decreased compared to the control group. Furthermore, NSCs inhibited the iNOS expression of BMDMs and the release of inflammatory factors by macrophages in vitro (p < 0.05). These results suggest that NSC transplantation could modulate SCI-induced inflammatory responses and enhance neurological function after SCI via reducing M1 macrophage activation and infiltrating neutrophils. Thus, this study provides a new insight into the mechanisms responsible for the anti-inflammatory effect of NSC transplantation after SCI.
Identifier: FSU_pmch_27563878 (IID), 10.3390/ijms17091380 (DOI), PMC5037660 (PMCID), 27563878 (RID), 27563878 (EID), ijms17091380 (PII)
Keywords: Inflammatory cytokine, Macrophage, Neural stem cells, Spinal cord injury
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5037660.
Subject(s): Animals
Cells, Cultured
Female
Immunohistochemistry
Interleukin-10/metabolism
Interleukin-12/metabolism
Interleukin-1beta/metabolism
Interleukin-6/metabolism
Macrophages/cytology
Macrophages/physiology
Mice
Mice, Inbred C57BL
Neural Stem Cells/cytology
Neural Stem Cells/physiology
Neutrophils/cytology
Neutrophils/physiology
Spinal Cord Injuries/immunology
Spinal Cord Injuries/therapy
Stem Cell Transplantation/methods
Tumor Necrosis Factor-alpha/metabolism
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_27563878
Owner Institution: FSU
Is Part Of: International journal of molecular sciences.
1422-0067
Issue: iss. 9, vol. 17

Choose the citation style.
Cheng, Z., Zhu, W., Cao, K., Wu, F., Li, J., Wang, G., … He, X. (2016). Anti-Inflammatory Mechanism of Neural Stem Cell Transplantation in Spinal Cord Injury. International Journal Of Molecular Sciences. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_27563878