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tunable artificial circadian clock in clock-defective mice.

Title: A tunable artificial circadian clock in clock-defective mice.
Name(s): D'Alessandro, Matthew, author
Beesley, Stephen, author
Kim, Jae Kyoung, author
Chen, Rongmin, author
Abich, Estela, author
Cheng, Wayne, author
Yi, Paul, author
Takahashi, Joseph S, author
Lee, Choogon, author
Type of Resource: text
Genre: Journal Article
Date Issued: 2015-11-30
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Self-sustaining oscillations are essential for diverse physiological functions such as the cell cycle, insulin secretion and circadian rhythms. Synthetic oscillators using biochemical feedback circuits have been generated in cell culture. These synthetic systems provide important insight into design principles for biological oscillators, but have limited similarity to physiological pathways. Here we report the generation of an artificial, mammalian circadian clock in vivo, capable of generating robust, tunable circadian rhythms. In mice deficient in Per1 and Per2 genes (thus lacking circadian rhythms), we artificially generate PER2 rhythms and restore circadian sleep/wake cycles with an inducible Per2 transgene. Our artificial clock is tunable as the period and phase of the rhythms can be modulated predictably. This feature, and other design principles of our work, might enhance the study and treatment of circadian dysfunction and broader aspects of physiology involving biological oscillators.
Identifier: FSU_pmch_26617050 (IID), 10.1038/ncomms9587 (DOI), PMC4674671 (PMCID), 26617050 (RID), 26617050 (EID), ncomms9587 (PII)
Grant Number: R01 DK090730, DK-090730
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at
Subject(s): Animals
Chronobiology Disorders/genetics
Chronobiology Disorders/metabolism
Chronobiology Disorders/physiopathology
Circadian Clocks
Disease Models, Animal
Mice, Inbred C57BL
Mice, Knockout
Period Circadian Proteins/deficiency
Period Circadian Proteins/genetics
Persistent Link to This Record:
Host Institution: FSU
Is Part Of: Nature communications.
Issue: vol. 6

Choose the citation style.
D'Alessandro, M., Beesley, S., Kim, J. K., Chen, R., Abich, E., Cheng, W., … Lee, C. (2015). A tunable artificial circadian clock in clock-defective mice. Nature Communications. Retrieved from