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New methods for investigation of neuronal migration in embryonic brain explants.

Title: New methods for investigation of neuronal migration in embryonic brain explants.
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Name(s): Nery, Flávia C, author
da Hora, Cintia C, author
Yaqub, Uzma, author
Zhang, Xuan, author
McCarthy, Deirdre M, author
Bhide, Pradeep G, author
Irimia, Daniel, author
Breakefield, Xandra O, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2015-01-15
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Proper migration of neurons is essential for the formation and normal functioning of the nervous system. Defects in neuronal migration underlie a number of neurologic diseases in humans. Although cell migration is crucial for neural development, molecular mechanisms guiding neuronal migration remain to be elucidated fully. Newborn neurons from the embryonic medial ganglionic eminence (MGE) migrate a long distance dorsally in the developing brain, giving rise to several types of interneurons in the neocortex. In this study, we developed an immunocytochemistry (ICC) protocol to stain neurons migrating out of the MGE explant embedded in Matrigel. We also established a protocol to efficiently transfect cells in MGE explants, achieving a transduction efficiency of more than 30%. In addition, we developed microfluidic chambers for explants that allow visualization of the vectorial migration of individual neurons from mouse embryonic MGE explants. Our microfluidic system allows monitoring of the distribution of cellular organelles (e.g. Golgi) within migrating neurons which have been stained with commercial molecular dyes or transfected with adeno-associated virus (AAV) expressing reporter proteins. These methods provide new paradigms to study neuronal migration in real-time.
Identifier: FSU_pmch_25291524 (IID), 10.1016/j.jneumeth.2014.09.028 (DOI), PMC4268085 (PMCID), 25291524 (RID), 25291524 (EID), S0165-0270(14)00353-7 (PII)
Keywords: MGE explant, Microfluidic device, Neuron migration, Transduction
Grant Number: P50 NS037409, P30 NS045776, R01 GM092804, GM092804, EB002503, P41 EB002503
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4268085.
Subject(s): Animals
Antigens/metabolism
Cell Movement/physiology
Dependovirus/genetics
Embryo, Mammalian
Female
Green Fluorescent Proteins/genetics
Green Fluorescent Proteins/metabolism
In Vitro Techniques
Male
Median Eminence/cytology
Mice
Mice, Inbred C57BL
Microfluidic Analytical Techniques
Neurons/physiology
Organ Culture Techniques
Transduction, Genetic
Tubulin/metabolism
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_25291524
Owner Institution: FSU
Is Part Of: Journal of neuroscience methods.
1872-678X
Issue: vol. 239

Choose the citation style.
Nery, F. C., Da Hora, C. C., Yaqub, U., Zhang, X., McCarthy, D. M., Bhide, P. G., … Breakefield, X. O. (2015). New methods for investigation of neuronal migration in embryonic brain explants. Journal Of Neuroscience Methods. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_25291524