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Myelin basic protein induces neuron-specific toxicity by directly damaging the neuronal plasma membrane.

Title: Myelin basic protein induces neuron-specific toxicity by directly damaging the neuronal plasma membrane.
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Name(s): Zhang, Jie, author
Sun, Xin, author
Zheng, Sixin, author
Liu, Xiao, author
Jin, Jinghua, author
Ren, Yi, author
Luo, Jianhong, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2014-09-25
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: The central nervous system (CNS) insults may cause massive demyelination and lead to the release of myelin-associated proteins including its major component myelin basic protein (MBP). MBP is reported to induce glial activation but its effect on neurons is still little known. Here we found that MBP specifically bound to the extracellular surface of the neuronal plasma membrane and induced neurotoxicity in vitro. This effect of MBP on neurons was basicity-dependent because the binding was blocked by acidic lipids and competed by other basic proteins. Further studies revealed that MBP induced damage to neuronal membrane integrity and function by depolarizing the resting membrane potential, increasing the permeability to cations and other molecules, and decreasing the membrane fluidity. At last, artificial liposome vesicle assay showed that MBP directly disturbed acidic lipid bilayer and resulted in increased membrane permeability. These results revealed that MBP induces neurotoxicity through its direct interaction with acidic components on the extracellular surface of neuronal membrane, which may suggest a possible contribution of MBP to the pathogenesis in the CNS disorders with myelin damage.
Identifier: FSU_pmch_25255088 (IID), 10.1371/journal.pone.0108646 (DOI), PMC4177931 (PMCID), 25255088 (RID), 25255088 (EID), PONE-D-14-18395 (PII)
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4177931.
Subject(s): Animals
Cations/metabolism
Cell Membrane/drug effects
Cell Membrane/metabolism
Cell Membrane Permeability/drug effects
Female
Lipid Bilayers/chemistry
Lipid Bilayers/metabolism
Male
Membrane Potentials/drug effects
Myelin Basic Protein/metabolism
Myelin Basic Protein/toxicity
Neurons/drug effects
Neurons/physiology
Protein Binding
Pyramidal Cells/drug effects
Pyramidal Cells/physiology
Rats
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_25255088
Owner Institution: FSU
Is Part Of: PloS one.
1932-6203
Issue: iss. 9, vol. 9

Choose the citation style.
Zhang, J., Sun, X., Zheng, S., Liu, X., Jin, J., Ren, Y., & Luo, J. (2014). Myelin basic protein induces neuron-specific toxicity by directly damaging the neuronal plasma membrane. Plos One. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_25255088