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Microglia from neurogenic and non-neurogenic regions display differential proliferative potential and neuroblast support.

Title: Microglia from neurogenic and non-neurogenic regions display differential proliferative potential and neuroblast support.
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Name(s): Marshall, Gregory P, author
Deleyrolle, Loic P, author
Reynolds, Brent A, author
Steindler, Dennis A, author
Laywell, Eric D, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2014-07-16
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Microglia isolated from the neurogenic subependymal zone (SEZ) and hippocampus (HC) are capable of massive in vitro population expansion that is not possible with microglia isolated from non-neurogenic regions. We asked if this regional heterogeneity in microglial proliferative capacity is cell intrinsic, or is conferred by interaction with respective neurogenic or non-neurogenic niches. By combining SEZ and cerebral cortex (CTX) primary tissue dissociates to generate heterospatial cultures, we find that exposure to the SEZ environment does not enhance CTX microglia expansion; however, the CTX environment exerts a suppressive effect on SEZ microglia expansion. Furthermore, addition of purified donor SEZ microglia to either CTX- or SEZ-derived cultures suppresses the expansion of host microglia, while the addition of donor CTX microglia enhances the over-all microglia yield. These data suggest that SEZ and CTX microglia possess intrinsic, spatially restricted characteristics that are independent of their in vitro environment, and that they represent unique and functionally distinct populations. Finally, we determined that the repeated supplementation of neurogenic SEZ cultures with expanded SEZ microglia allows for sustained levels of inducible neurogenesis, provided that the ratio of microglia to total cells remains within a fairly narrow range.
Identifier: FSU_pmch_25076873 (IID), 10.3389/fncel.2014.00180 (DOI), PMC4100441 (PMCID), 25076873 (RID), 25076873 (EID)
Keywords: Functional heterogeneity, Inducible neurogenesis, Microglia, Proliferation, Subependymal zone
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100441.
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_25076873
Owner Institution: FSU
Is Part Of: Frontiers in cellular neuroscience.
1662-5102
Issue: vol. 8

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Marshall, G. P., Deleyrolle, L. P., Reynolds, B. A., Steindler, D. A., & Laywell, E. D. (2014). Microglia from neurogenic and non-neurogenic regions display differential proliferative potential and neuroblast support. Frontiers In Cellular Neuroscience. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_25076873