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Drosophila pericentrin requires interaction with calmodulin for its function at centrosomes and neuronal basal bodies but not at sperm basal bodies.

Title: Drosophila pericentrin requires interaction with calmodulin for its function at centrosomes and neuronal basal bodies but not at sperm basal bodies.
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Name(s): Galletta, Brian J, author
Guillen, Rodrigo X, author
Fagerstrom, Carey J, author
Brownlee, Chris W, author
Lerit, Dorothy A, author
Megraw, Timothy L, author
Rogers, Gregory C, author
Rusan, Nasser M, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2014-09-15
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Pericentrin is a critical centrosomal protein required for organizing pericentriolar material (PCM) in mitosis. Mutations in pericentrin cause the human genetic disorder Majewski/microcephalic osteodysplastic primordial dwarfism type II, making a detailed understanding of its regulation extremely important. Germaine to pericentrin's function in organizing PCM is its ability to localize to the centrosome through the conserved C-terminal PACT domain. Here we use Drosophila pericentrin-like-protein (PLP) to understand how the PACT domain is regulated. We show that the interaction of PLP with calmodulin (CaM) at two highly conserved CaM-binding sites in the PACT domain controls the proper targeting of PLP to the centrosome. Disrupting the PLP-CaM interaction with single point mutations renders PLP inefficient in localizing to centrioles in cultured S2 cells and Drosophila neuroblasts. Although levels of PCM are unaffected, it is highly disorganized. We also demonstrate that basal body formation in the male testes and the production of functional sperm does not rely on the PLP-CaM interaction, whereas production of functional mechanosensory neurons does.
Identifier: FSU_pmch_25031429 (IID), 10.1091/mbc.E13-10-0617 (DOI), PMC4161505 (PMCID), 25031429 (RID), 25031429 (EID), mbc.E13-10-0617 (PII)
Grant Number: P30 CA023074, R01GM068756, ZIA HL006126, 1ZIAHL006126, P50 CA095060, ZIA HL006104-03, P30CA23074, R01 GM068756, P50CA95060
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4161505.
Subject(s): Amino Acid Sequence
Animals
Basal Bodies/metabolism
Calmodulin/metabolism
Cell Line
Centrioles/physiology
Drosophila Proteins/metabolism
Drosophila melanogaster
Male
Mechanotransduction, Cellular
Molecular Sequence Data
Neurons/physiology
Protein Interaction Domains and Motifs
Protein Transport
Sperm Motility
Spermatogenesis
Spermatozoa/physiology
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_25031429
Owner Institution: FSU
Is Part Of: Molecular biology of the cell.
1939-4586
Issue: iss. 18, vol. 25

Choose the citation style.
Galletta, B. J., Guillen, R. X., Fagerstrom, C. J., Brownlee, C. W., Lerit, D. A., Megraw, T. L., … Rusan, N. M. (2014). Drosophila pericentrin requires interaction with calmodulin for its function at centrosomes and neuronal basal bodies but not at sperm basal bodies. Molecular Biology Of The Cell. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_25031429