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interactive network of long non-coding RNAs facilitates the Drosophila sex determination decision.

Title: An interactive network of long non-coding RNAs facilitates the Drosophila sex determination decision.
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Name(s): Mulvey, Brett B, author
Olcese, Ursula, author
Cabrera, Janel R, author
Horabin, Jamila I, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2014-09-01
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Genome analysis in several eukaryotes shows a surprising number of transcripts which do not encode conventional messenger RNAs. Once considered noise, these non-coding RNAs (ncRNAs) appear capable of controlling gene expression by various means. We find that Drosophila sex determination, specifically the master-switch gene Sex-lethal (Sxl), is regulated by long ncRNAs (>200nt). The lncRNAs influence the dose sensitive establishment promoter of Sxl, SxlPe, which must be activated to specify female sex. They are primarily from two regions, R1 and R2, upstream of SxlPe and show a dynamic developmental profile. Of the four lncRNA strands only one, R2 antisense, has its peak coincident with SxlPe transcription, suggesting that it may promote activation. Indeed, its expression is regulated by the X chromosome counting genes, whose dose determines whether SxlPe is transcribed. Transgenic lines which ectopically express each of the lncRNAs show they can act in trans, not only impacting the process of sex determination but also altering the levels of the other lncRNAs. Generally, expression of R1 is negative whereas R2 is positive to females. This ectopic expression also results in a change in the local chromatin marks, affecting the timing and strength of SxlPe transcription. The chromatin marks are those deposited by the Polycomb and trithorax groups of chromatin modifying proteins, which we find bind to the lncRNAs. We suggest that the increasing numbers of non-coding transcripts being identified are a harbinger of interacting networks similar to the one we describe.
Identifier: FSU_pmch_24954180 (IID), 10.1016/j.bbagrm.2014.06.007 (DOI), PMC4134978 (PMCID), 24954180 (RID), 24954180 (EID), S1874-9399(14)00159-X (PII)
Keywords: Drosophila, Sex determination, Sex-lethal, Transcription, LncRNA
Grant Number: R01 GM085165
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4134978.
Subject(s): Animals
Chromosomal Proteins, Non-Histone/genetics
Drosophila/genetics
Drosophila Proteins/genetics
Mutation
Polycomb-Group Proteins/genetics
Promoter Regions, Genetic
RNA, Long Noncoding/physiology
RNA-Binding Proteins/genetics
Sex Determination Processes
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_24954180
Owner Institution: FSU
Is Part Of: Biochimica et biophysica acta.
0006-3002
Issue: iss. 9, vol. 1839

Choose the citation style.
Mulvey, B. B., Olcese, U., Cabrera, J. R., & Horabin, J. I. (2014). An interactive network of long non-coding RNAs facilitates the Drosophila sex determination decision. Biochimica Et Biophysica Acta. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_24954180