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RFX7 is required for the formation of cilia in the neural tube.

Title: RFX7 is required for the formation of cilia in the neural tube.
Name(s): Manojlovic, Zarko, author
Earwood, Ryan, author
Kato, Akiko, author
Stefanovic, Branko, author
Kato, Yoichi, author
Type of Resource: text
Genre: Journal Article
Date Issued: 2014-05-01
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Regulatory Factor X (RFX) transcription factors are important for development and are likely involved in the pathogenesis of serious human diseases including ciliopathies. While seven RFX genes have been identified in vertebrates and several RFX transcription factors have been reported to be regulators of ciliogenesis, the role of RFX7 in development including ciliogenesis is not known. Here we show that RFX7 in Xenopus laevis is expressed in the neural tube, eye, otic vesicles, and somites. Knockdown of RFX7 in Xenopus embryos resulted in a defect of ciliogenesis in the neural tube and failure of neural tube closure. RFX7 controlled the formation of cilia by regulating the expression of RFX4 gene, which has been reported to be required for ciliogenesis in the neural tube. Moreover, ectopic expression of Foxj1, which is a master regulator of motile cilia formation, suppressed the expression of RFX4 but not RFX7. Taken together, RFX7 plays an important role in the process of neural tube closure at the top of the molecular cascade which controls ciliogenesis in the neural tube.
Identifier: FSU_pmch_24530844 (IID), 10.1016/j.mod.2014.02.001 (DOI), PMC3976564 (PMCID), 24530844 (RID), 24530844 (EID), S0925-4773(14)00008-2 (PII)
Keywords: Cilia, Neural tube closure, RFX, Xenopus laevis
Grant Number: AA019845, DK059466, GM087641, R01 DK059466, F31 AA019845, R15 GM087641
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at
Subject(s): Animals
Base Sequence
DNA-Binding Proteins/genetics
Forkhead Transcription Factors/genetics
Gene Expression Regulation, Developmental/genetics
Molecular Sequence Data
Neural Tube/embryology
Regulatory Factor X Transcription Factors
Transcription Factors/genetics
Xenopus Proteins/genetics
Xenopus laevis
Persistent Link to This Record:
Owner Institution: FSU
Is Part Of: Mechanisms of development.
Issue: vol. 132

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Manojlovic, Z., Earwood, R., Kato, A., Stefanovic, B., & Kato, Y. (2014). RFX7 is required for the formation of cilia in the neural tube. Mechanisms Of Development. Retrieved from