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Melatonin in the mammalian olfactory bulb.

Title: Melatonin in the mammalian olfactory bulb.
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Name(s): Corthell, J T, author
Olcese, J, author
Trombley, P Q, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2014-03-07
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Melatonin is a neurohormone associated with circadian rhythms. A diurnal rhythm in olfactory sensitivity has been previously reported and melatonin receptor mRNAs have been observed in the olfactory bulb, but the effects of melatonin in the olfactory bulb have not been explored. First, we corroborated data from a previous study that identified melatonin receptor messenger RNAs in the olfactory bulb. We then investigated whether melatonin treatment would affect cells in the olfactory bulbs of rats. Using a combination of polymerase chain reaction (PCR), quantitative PCR (qPCR), cell culture, and electrophysiology, we discovered that melatonin receptors and melatonin synthesis enzymes were present in the olfactory bulb and we observed changes in connexin43 protein, GluR1 mRNA, GluR2 mRNA, Per1 mRNA, Cry2 mRNA, and K(+) currents in response to 2-iodomelatonin. Via qPCR, we observed that messenger RNAs encoding melatonin receptors and melatonin biosynthesis enzymes fluctuated in the olfactory bulb across 24h. Together, these data show that melatonin receptors are present in the olfactory bulb and likely affect olfactory function. Additionally, these data suggest that melatonin may be locally synthesized in the olfactory bulb.
Identifier: FSU_pmch_24365461 (IID), 10.1016/j.neuroscience.2013.12.033 (DOI), PMC3939688 (PMCID), 24365461 (RID), 24365461 (EID), S0306-4522(13)01056-7 (PII)
Keywords: GluR2, Juxtaglomerular, Melatonin, Olfaction, Potassium, Receptor
Grant Number: T32 DC000044, T32 DC00044
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3939688.
Subject(s): Animals
Astrocytes/metabolism
Cells, Cultured
Circadian Rhythm
Female
In Vitro Techniques
Male
Melatonin/metabolism
Mice
Mice, Knockout
Neurons/metabolism
Olfactory Bulb/enzymology
Olfactory Bulb/metabolism
Potassium/metabolism
RNA, Messenger/metabolism
Rats
Rats, Sprague-Dawley
Receptor, Melatonin, MT1/genetics
Receptor, Melatonin, MT1/metabolism
Receptor, Melatonin, MT2/genetics
Receptor, Melatonin, MT2/metabolism
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_24365461
Owner Institution: FSU
Is Part Of: Neuroscience.
1873-7544
Issue: vol. 261

Choose the citation style.
Corthell, J. T., Olcese, J., & Trombley, P. Q. (2014). Melatonin in the mammalian olfactory bulb. Neuroscience. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_24365461