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replication initiation protein Sld2 regulates helicase assembly.

Title: The replication initiation protein Sld2 regulates helicase assembly.
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Name(s): Bruck, Irina, author
Kaplan, Daniel L, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2014-01-24
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Assembly of the Cdc45-Mcm2-7-GINS (CMG) replicative helicase complex must be regulated to ensure that DNA unwinding is coupled with DNA synthesis. Sld2 is required for the initiation of DNA replication in budding yeast. We identified a mutant of Sld2, Sld2-m1,4, that is specifically defective in Mcm2-7 binding. When this sld2-m1,4 mutant is expressed, cells exhibit severe inhibition of DNA replication. Furthermore, the CMG complex assembles prematurely in G1 in mutant cells, but not wild-type cells. These data suggest that Sld2 binding to Mcm2-7 is essential to block the inappropriate formation of a CMG helicase complex in G1. We also study a mutant of Sld2 that is defective in binding DNA, sld2-DNA, and find that sld2-DNA cells exhibit no GINS-Mcm2-7 interaction. These data suggest that Sld2 association with DNA is required for CMG assembly in S phase.
Identifier: FSU_pmch_24307213 (IID), 10.1074/jbc.M113.532085 (DOI), PMC3900945 (PMCID), 24307213 (RID), 24307213 (EID), M113.532085 (PII)
Keywords: CDK (Cyclin-dependent Kinase), Cell Cycle, DNA Helicase, DNA Replication, DNA-binding Protein
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3900945.
Subject(s): Cell Cycle Proteins/genetics
Cell Cycle Proteins/metabolism
DNA Replication/physiology
DNA, Fungal/biosynthesis
DNA, Fungal/genetics
DNA-Binding Proteins/genetics
DNA-Binding Proteins/metabolism
Minichromosome Maintenance Complex Component 7/genetics
Minichromosome Maintenance Complex Component 7/metabolism
Minichromosome Maintenance Proteins/genetics
Minichromosome Maintenance Proteins/metabolism
Mutation
Nuclear Proteins/genetics
Nuclear Proteins/metabolism
Protein Binding/physiology
S Phase/physiology
Saccharomyces cerevisiae/genetics
Saccharomyces cerevisiae/metabolism
Saccharomyces cerevisiae Proteins/genetics
Saccharomyces cerevisiae Proteins/metabolism
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_24307213
Owner Institution: FSU
Is Part Of: The Journal of biological chemistry.
1083-351X
Issue: iss. 4, vol. 289

Choose the citation style.
Bruck, I., & Kaplan, D. L. (2014). The replication initiation protein Sld2 regulates helicase assembly. The Journal Of Biological Chemistry. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_24307213