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Male-specific Fruitless isoforms have different regulatory roles conferred by distinct zinc finger DNA binding domains.

Title: Male-specific Fruitless isoforms have different regulatory roles conferred by distinct zinc finger DNA binding domains.
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Name(s): Dalton, Justin E, author
Fear, Justin M, author
Knott, Simon, author
Baker, Bruce S, author
McIntyre, Lauren M, author
Arbeitman, Michelle N, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2013-09-27
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Drosophila melanogaster adult males perform an elaborate courtship ritual to entice females to mate. fruitless (fru), a gene that is one of the key regulators of male courtship behavior, encodes multiple male-specific isoforms (Fru(M)). These isoforms vary in their carboxy-terminal zinc finger domains, which are predicted to facilitate DNA binding. By over-expressing individual Fru(M) isoforms in fru-expressing neurons in either males or females and assaying the global transcriptional response by RNA-sequencing, we show that three Fru(M) isoforms have different regulatory activities that depend on the sex of the fly. We identified several sets of genes regulated downstream of Fru(M) isoforms, including many annotated with neuronal functions. By determining the binding sites of individual Fru(M) isoforms using SELEX we demonstrate that the distinct zinc finger domain of each Fru(M) isoforms confers different DNA binding specificities. A genome-wide search for these binding site sequences finds that the gene sets identified as induced by over-expression of Fru(M) isoforms in males are enriched for genes that contain the binding sites. An analysis of the chromosomal distribution of genes downstream of Fru(M) shows that those that are induced and repressed in males are highly enriched and depleted on the X chromosome, respectively. This study elucidates the different regulatory and DNA binding activities of three Fru(M) isoforms on a genome-wide scale and identifies genes regulated by these isoforms. These results add to our understanding of sex chromosome biology and further support the hypothesis that in some cell-types genes with male-biased expression are enriched on the X chromosome.
Identifier: FSU_pmch_24074028 (IID), 10.1186/1471-2164-14-659 (DOI), PMC3852243 (PMCID), 24074028 (RID), 24074028 (EID), 1471-2164-14-659 (PII)
Grant Number: R01 GM102227, R01 MH091561, R01GM073039, R01MH091561, R01 GM073039
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3852243.
Subject(s): Animals
Base Sequence
Binding Sites
Chromosomes, Insect/genetics
DNA-Binding Proteins/chemistry
DNA-Binding Proteins/genetics
DNA-Binding Proteins/metabolism
Drosophila Proteins/chemistry
Drosophila Proteins/genetics
Drosophila Proteins/metabolism
Drosophila melanogaster/genetics
Drosophila melanogaster/metabolism
Female
Gene Expression Regulation
Male
Molecular Sequence Data
Nerve Tissue Proteins/chemistry
Nerve Tissue Proteins/genetics
Nerve Tissue Proteins/metabolism
Neurons/metabolism
Protein Isoforms/chemistry
Protein Isoforms/genetics
Protein Isoforms/metabolism
Protein Structure, Tertiary
Sex Characteristics
Transcription Factors/chemistry
Transcription Factors/genetics
Transcription Factors/metabolism
Zinc Fingers
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_24074028
Owner Institution: FSU
Is Part Of: BMC genomics.
1471-2164
Issue: vol. 14

Choose the citation style.
Dalton, J. E., Fear, J. M., Knott, S., Baker, B. S., McIntyre, L. M., & Arbeitman, M. N. (2013). Male-specific Fruitless isoforms have different regulatory roles conferred by distinct zinc finger DNA binding domains. Bmc Genomics. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_24074028