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δ/ω-Plectoxin-Pt1a

Title: δ/ω-Plectoxin-Pt1a: an excitatory spider toxin with actions on both Ca(2+) and Na(+) channels.
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Name(s): Zhou, Yi, author
Zhao, Mingli, author
Fields, Gregg B, author
Wu, Chun-Fang, author
Branton, W Dale, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2013-05-14
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: The venom of spider Plectreurys tristis contains a variety of peptide toxins that selectively target neuronal ion channels. O-palmitoylation of a threonine or serine residue, along with a characteristic and highly constrained disulfide bond structure, are hallmarks of a family of toxins found in this venom. Here, we report the isolation and characterization of a new toxin, δ/ω-plectoxin-Pt1a, from this spider venom. It is a 40 amino acid peptide containing an O-palmitoylated Ser-39. Analysis of δ/ω-plectoxin-Pt1a cDNA reveals a small precursor containing a secretion signal sequence, a 14 amino acid N-terminal propeptide, and a C-terminal amidation signal. The biological activity of δ/ω-plectoxin-Pt1a is also unique. It preferentially blocks a subset of Ca(2+) channels that is apparently not required for neurotransmitter release; decreases threshold for Na(+) channel activation; and slows Na(+) channel inactivation. As δ/ω-plectoxin-Pt1a enhances synaptic transmission by prolonging presynaptic release of neurotransmitter, its effects on Na(+) and Ca(2+) channels may act synergistically to sustain the terminal excitability.
Identifier: FSU_pmch_23691198 (IID), 10.1371/journal.pone.0064324 (DOI), PMC3653879 (PMCID), 23691198 (RID), 23691198 (EID), PONE-D-13-04611 (PII)
Grant Number: NS50355
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3653879.
Subject(s): Amino Acid Sequence
Animals
Base Sequence
Calcium Channel Blockers/chemistry
Calcium Channel Blockers/toxicity
Calcium Channels/metabolism
Drosophila/cytology
Lipoylation
Molecular Sequence Data
Neurons/cytology
Neurons/drug effects
Neurons/metabolism
Neurotoxins/chemistry
Neurotoxins/genetics
Neurotoxins/toxicity
Sodium Channel Blockers/chemistry
Sodium Channel Blockers/toxicity
Sodium Channels/metabolism
Spider Venoms/chemistry
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_23691198
Owner Institution: FSU
Is Part Of: PloS one.
1932-6203
Issue: iss. 5, vol. 8

Choose the citation style.
Zhou, Y., Zhao, M., Fields, G. B., Wu, C. -F., & Branton, W. D. (2013). δ/ω-Plectoxin-Pt1a: an excitatory spider toxin with actions on both Ca(2+) and Na(+) channels. Plos One. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_23691198