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Slk19 clusters kinetochores and facilitates chromosome bipolar attachment.

Title: Slk19 clusters kinetochores and facilitates chromosome bipolar attachment.
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Name(s): Richmond, Daniel, author
Rizkallah, Raed, author
Liang, Fengshan, author
Hurt, Myra M, author
Wang, Yanchang, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2013-03-01
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: In all eukaryotic cells, DNA is packaged into multiple chromosomes that are linked to microtubules through a large protein complex called a kinetochore. Previous data show that the kinetochores are clustered together during most of the cell cycle, but the mechanism and the biological significance of kinetochore clustering are unknown. As a kinetochore protein in budding yeast, the role of Slk19 in the stability of the anaphase spindle has been well studied, but its function in chromosome segregation has remained elusive. Here we show that Slk19 is required for kinetochore clustering when yeast cells are treated with the microtubule-depolymerizing agent nocodazole. We further find that slk19Δ mutant cells exhibit delayed kinetochore capture and chromosome bipolar attachment after the disruption of the kinetochore-microtubule interaction by nocodazole, which is likely attributed to defective kinetochore clustering. In addition, we show that Slk19 interacts with itself, suggesting that the dimerization of Slk19 may mediate the interaction between kinetochores for clustering. Therefore Slk19 likely acts as kinetochore glue that clusters kinetochores to facilitate efficient and faithful chromosome segregation.
Identifier: FSU_pmch_23283988 (IID), 10.1091/mbc.E12-07-0552 (DOI), PMC3583661 (PMCID), 23283988 (RID), 23283988 (EID), mbc.E12-07-0552 (PII)
Grant Number: R15 GM097326, R15GM097326-01
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3583661.
Subject(s): Anaphase/genetics
Chromosome Segregation/drug effects
Chromosome Segregation/genetics
Chromosomes/genetics
Chromosomes/ultrastructure
DNA/drug effects
DNA/genetics
Kinetochores/drug effects
Kinetochores/ultrastructure
Microtubule-Associated Proteins/genetics
Microtubules/drug effects
Microtubules/genetics
Mitosis/genetics
Mutation
Nocodazole/pharmacology
Saccharomyces cerevisiae/genetics
Saccharomyces cerevisiae Proteins/genetics
Spindle Apparatus/drug effects
Spindle Apparatus/genetics
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_23283988
Owner Institution: FSU
Is Part Of: Molecular biology of the cell.
1939-4586
Issue: iss. 5, vol. 24

Choose the citation style.
Richmond, D., Rizkallah, R., Liang, F., Hurt, M. M., & Wang, Y. (2013). Slk19 clusters kinetochores and facilitates chromosome bipolar attachment. Molecular Biology Of The Cell. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_23283988