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Phosphorylation of the transcription factor YY1 by CK2α prevents cleavage by caspase 7 during apoptosis.

Title: Phosphorylation of the transcription factor YY1 by CK2α prevents cleavage by caspase 7 during apoptosis.
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Name(s): Riman, Sarah, author
Rizkallah, Raed, author
Kassardjian, Ari, author
Alexander, Karen E, author
Lüscher, Bernhard, author
Hurt, Myra M, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2012-02-01
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: In this report, we describe the phosphorylation of Yin Yang 1 (YY1) in vitro and in vivo by CK2α (casein kinase II), a multifunctional serine/threonine protein kinase. YY1 is a ubiquitously expressed multifunctional zinc finger transcription factor implicated in regulation of many cellular and viral genes. The products of these genes are associated with cell growth, the cell cycle, development, and differentiation. Numerous studies have linked YY1 to tumorigenesis and apoptosis. YY1 is a target for cleavage by caspases in vitro and in vivo as well, but very little is known about the mechanisms that regulate its cleavage during apoptosis. Here, we identify serine 118 in the transactivation domain of YY1 as the site of CK2α phosphorylation, proximal to a caspase 7 cleavage site. CK2α inhibitors, as well as knockdown of CK2α by small interfering RNA, reduce S118 phosphorylation in vivo and enhance YY1 cleavage under apoptotic conditions, whereas increased CK2α activity by overexpression in vivo elevates S118 phosphorylation. A serine-to-alanine substitution at serine 118 also increases the cleavage of YY1 during apoptosis compared to wild-type YY1. Taken together, we have discovered a regulatory link between YY1 phosphorylation at serine 118 and regulation of its cleavage during programmed cell death.
Identifier: FSU_pmch_22184066 (IID), 10.1128/MCB.06466-11 (DOI), PMC3272974 (PMCID), 22184066 (RID), 22184066 (EID), MCB.06466-11 (PII)
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3272974.
Subject(s): Amino Acid Sequence
Amino Acid Substitution
Apoptosis/physiology
Base Sequence
Binding Sites
Casein Kinase II/metabolism
Caspase 7/metabolism
DNA Primers/genetics
HEK293 Cells
HeLa Cells
Humans
Models, Biological
Models, Molecular
Molecular Sequence Data
Mutagenesis, Site-Directed
Phosphorylation
Protein Structure, Tertiary
Recombinant Proteins/chemistry
Recombinant Proteins/genetics
Recombinant Proteins/metabolism
Serine/chemistry
YY1 Transcription Factor/chemistry
YY1 Transcription Factor/genetics
YY1 Transcription Factor/metabolism
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_22184066
Owner Institution: FSU
Is Part Of: Molecular and cellular biology.
1098-5549
Issue: iss. 4, vol. 32

Choose the citation style.
Riman, S., Rizkallah, R., Kassardjian, A., Alexander, K. E., Lüscher, B., & Hurt, M. M. (2012). Phosphorylation of the transcription factor YY1 by CK2α prevents cleavage by caspase 7 during apoptosis. Molecular And Cellular Biology. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_22184066