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Determining nuclear localization of alpha-synuclein in mouse brains.
| Title: | Determining nuclear localization of alpha-synuclein in mouse brains. |
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| Name(s): |
Huang, Z, author Xu, Z, author Wu, Y, author Zhou, Y, author |
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| Type of Resource: | text | |
| Genre: |
Journal Article Text |
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| Date Issued: | 2011-12-29 | |
| Physical Form: |
computer online resource |
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| Extent: | 1 online resource | |
| Language(s): | English | |
| Abstract/Description: | Alpha-synuclein (α-Syn) is a major component of Lewy bodies, abnormal protein aggregates that are present in neurons of patients with Parkinson's disease and other neurological disorders. Despite intensive investigation, the in vivo role of α-Syn in physiological and pathological processes is not fully understood. This study addresses a current debate on the nuclear localization of α-Syn protein in the brain. To assess the specificity of various α-Syn antibodies, we compared their staining patterns in wild-type mouse brains with that of the α-Syn knock-out mice. Among five different α-Syn antibodies tested here, two generated intensive nuclear staining throughout the normal mouse brain. However, nuclear staining by these two antibodies was also present in neurons of the α-Syn knock-out mice. This provides evidence that the nuclear signal is not specifically related to the presence of α-Syn, but it rather results from the cross-reactivity of the two antibodies to some unknown antigens in neuronal nuclei. In mouse brain neurons, endogenous α-Syn proteins are primarily localized to neuronal processes and nerve terminals but present only at low levels in the cell bodies. This is different from a generally uniform distribution of exogenously expressed α-Syn in both cytoplasm and nuclei of heterologous cells and suggests that the neuritic enrichment of α-Syn in neurons may be mediated by their specific interactions with certain structural or molecular components in the neuropil. | |
| Identifier: | FSU_pmch_22033456 (IID), 10.1016/j.neuroscience.2011.10.016 (DOI), PMC3237852 (PMCID), 22033456 (RID), 22033456 (EID), S0306-4522(11)01190-0 (PII) | |
| Grant Number: | R01 NS050355, R01 NS050355-05, NS50355 | |
| Publication Note: | This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3237852. | |
| Subject(s): |
Animals Antibodies Antibody Specificity Blotting, Western Brain/metabolism Cell Nucleus/metabolism Cross Reactions Fluorescent Antibody Technique Mice Mice, Inbred C57BL Mice, Knockout Neurons/metabolism alpha-Synuclein/metabolism |
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| Persistent Link to This Record: | http://purl.flvc.org/fsu/fd/FSU_pmch_22033456 | |
| Owner Institution: | FSU | |
| Is Part Of: |
Neuroscience. 1873-7544 |
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| Issue: | vol. 199 |
Huang, Z., Xu, Z., Wu, Y., & Zhou, Y. (2011). Determining nuclear localization of alpha-synuclein in mouse brains. Neuroscience. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_22033456
