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Determining nuclear localization of alpha-synuclein in mouse brains.

Title: Determining nuclear localization of alpha-synuclein in mouse brains.
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Name(s): Huang, Z, author
Xu, Z, author
Wu, Y, author
Zhou, Y, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2011-12-29
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Alpha-synuclein (α-Syn) is a major component of Lewy bodies, abnormal protein aggregates that are present in neurons of patients with Parkinson's disease and other neurological disorders. Despite intensive investigation, the in vivo role of α-Syn in physiological and pathological processes is not fully understood. This study addresses a current debate on the nuclear localization of α-Syn protein in the brain. To assess the specificity of various α-Syn antibodies, we compared their staining patterns in wild-type mouse brains with that of the α-Syn knock-out mice. Among five different α-Syn antibodies tested here, two generated intensive nuclear staining throughout the normal mouse brain. However, nuclear staining by these two antibodies was also present in neurons of the α-Syn knock-out mice. This provides evidence that the nuclear signal is not specifically related to the presence of α-Syn, but it rather results from the cross-reactivity of the two antibodies to some unknown antigens in neuronal nuclei. In mouse brain neurons, endogenous α-Syn proteins are primarily localized to neuronal processes and nerve terminals but present only at low levels in the cell bodies. This is different from a generally uniform distribution of exogenously expressed α-Syn in both cytoplasm and nuclei of heterologous cells and suggests that the neuritic enrichment of α-Syn in neurons may be mediated by their specific interactions with certain structural or molecular components in the neuropil.
Identifier: FSU_pmch_22033456 (IID), 10.1016/j.neuroscience.2011.10.016 (DOI), PMC3237852 (PMCID), 22033456 (RID), 22033456 (EID), S0306-4522(11)01190-0 (PII)
Grant Number: R01 NS050355, R01 NS050355-05, NS50355
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3237852.
Subject(s): Animals
Antibodies
Antibody Specificity
Blotting, Western
Brain/metabolism
Cell Nucleus/metabolism
Cross Reactions
Fluorescent Antibody Technique
Mice
Mice, Inbred C57BL
Mice, Knockout
Neurons/metabolism
alpha-Synuclein/metabolism
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_22033456
Owner Institution: FSU
Is Part Of: Neuroscience.
1873-7544
Issue: vol. 199

Choose the citation style.
Huang, Z., Xu, Z., Wu, Y., & Zhou, Y. (2011). Determining nuclear localization of alpha-synuclein in mouse brains. Neuroscience. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_22033456