You are here

NMDA receptor NR1 subunit is critically involved in the regulation of NMDA receptor activity by C-terminal Src kinase (Csk).

Title: The NMDA receptor NR1 subunit is critically involved in the regulation of NMDA receptor activity by C-terminal Src kinase (Csk).
14 views
3 downloads
Name(s): Fang, Xiao-Qian, author
Xu, Jindong, author
Feng, Shuang, author
Groveman, Bradley R, author
Lin, Shuang-Xiu, author
Yu, Xian-Min, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2011-02-01
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Previous studies have shown that Csk plays critical roles in the regulation of neural development, differentiation and glutamate-mediated synaptic plasticity. It has been found that Csk associates with the NR2A and 2B subunits of N-methyl-D-aspartate receptors (NMDARs) in a Src activity-dependent manner and serves as an intrinsic mechanism to provide a "brake" on the induction of long-term synaptic potentiation (LTP) mediated by NMDARs. In contrast to the NR2A and 2B subunits, no apparent tyrosine phosphorylation is found in the NR1 subunit of NMDARs. Here, we report that Csk can also associate with the NR1 subunit in a Src activity-dependent manner. The truncation of the NR1 subunit C-tail which contains only one tyrosine (Y837) significantly reduced the Csk association with the NR1-1a/NR2A receptor complex. Furthermore, we found that either the truncation of NR2A C-tail at aa 857 or the mutation of Y837 in the NR1-1a subunit to phenylalanine blocked the inhibition of NR1-1a/NR2A receptors induced by intracellular application of Csk. Thus, both the NR1 and NR2 subunits are required for the regulation of NMDAR activity by Csk.
Identifier: FSU_pmch_21113815 (IID), 10.1007/s11064-010-0330-0 (DOI), PMC3032389 (PMCID), 21113815 (RID), 21113815 (EID)
Grant Number: R01 NS053567, R01 NS053567-04, 5R01 NS053567-04
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3032389.
Subject(s): Animals
COS Cells
Cercopithecus aethiops
HEK293 Cells
Humans
Long-Term Potentiation/physiology
N-Methylaspartate/metabolism
Patch-Clamp Techniques
Protein-Tyrosine Kinases/genetics
Protein-Tyrosine Kinases/metabolism
Receptors, N-Methyl-D-Aspartate/genetics
Receptors, N-Methyl-D-Aspartate/metabolism
src-Family Kinases
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_21113815
Owner Institution: FSU
Is Part Of: Neurochemical research.
1573-6903
Issue: iss. 2, vol. 36

Choose the citation style.
Fang, X. -Q., Xu, J., Feng, S., Groveman, B. R., Lin, S. -X., & Yu, X. -M. (2011). The NMDA receptor NR1 subunit is critically involved in the regulation of NMDA receptor activity by C-terminal Src kinase (Csk). Neurochemical Research. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_21113815