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Intracellular regions of the Eag potassium channel play a critical role in generation of voltage-dependent currents.

Title: Intracellular regions of the Eag potassium channel play a critical role in generation of voltage-dependent currents.
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Name(s): Li, Yong, author
Liu, Xinqiu, author
Wu, Yuying, author
Xu, Zhe, author
Li, Hongqin, author
Griffith, Leslie C, author
Zhou, Yi, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2011-01-14
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Folding, assembly, and trafficking of ion channels are tightly controlled processes and are important for biological functions relevant to health and disease. Here, we report that functional expression of the Eag channel is temperature-sensitive by a mechanism that is independent of trafficking or surface targeting of the channel protein. Eag channels in cells grown at 37 °C exhibit voltage-evoked gating charge movements but fail to conduct K(+) ions. By mutagenesis and chimeric channel studies, we show that the N- and C-terminal regions are involved in controlling a step after movement of the voltage sensor, as well as in regulating biophysical properties of the Eag channel. Synthesis and assembly of Eag at high temperature disrupt the ability of these domains to carry out their function. These results suggest an important role of the intracellular regions in the generation of Eag currents.
Identifier: FSU_pmch_21059657 (IID), 10.1074/jbc.M110.184077 (DOI), PMC3020747 (PMCID), 21059657 (RID), 21059657 (EID), M110.184077 (PII)
Grant Number: R01 GM054408, R01 NS050355, GM54408, NS50355
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3020747.
Subject(s): Amino Acid Sequence
Animals
CHO Cells
Cricetinae
Cricetulus
Drosophila/physiology
Drosophila Proteins/chemistry
Drosophila Proteins/genetics
Drosophila Proteins/metabolism
Ether-A-Go-Go Potassium Channels/chemistry
Ether-A-Go-Go Potassium Channels/genetics
Ether-A-Go-Go Potassium Channels/metabolism
Ion Channel Gating/physiology
Membrane Potentials/physiology
Membrane Proteins/chemistry
Membrane Proteins/genetics
Membrane Proteins/metabolism
Molecular Sequence Data
Mutagenesis
Potassium/metabolism
Protein Folding
Protein Structure, Tertiary
Protein Transport/physiology
Recombinant Proteins/chemistry
Recombinant Proteins/genetics
Recombinant Proteins/metabolism
Temperature
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_21059657
Owner Institution: FSU
Is Part Of: The Journal of biological chemistry.
1083-351X
Issue: iss. 2, vol. 286

Choose the citation style.
Li, Y., Liu, X., Wu, Y., Xu, Z., Li, H., Griffith, L. C., & Zhou, Y. (2011). Intracellular regions of the Eag potassium channel play a critical role in generation of voltage-dependent currents. The Journal Of Biological Chemistry. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_21059657