You are here

Intracellular linkers are involved in Mg2+-dependent modulation of the Eag potassium channel.

Title: Intracellular linkers are involved in Mg2+-dependent modulation of the Eag potassium channel.
16 views
3 downloads
Name(s): Liu, Xinqiu, author
Wu, Yuying, author
Zhou, Yi, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2010-07-01
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Modulation of activation kinetics by divalent ions is one of the characteristic features of Eag channels. Here, we report that Mg(2+)-dependent deceleration of Eag channel activation is significantly attenuated by a G297E mutation, which exhibits a gain-of-function phenotype in Drosophila by suppressing the effect of shaker mutation on behavior and neuronal excitability. The G297 residue is located in the intracellular linker of transmembrane segments S2 and S3, and is thus not involved in direct binding of Mg(2+) ions. Moreover, mutation of the only positively charged residue in the other intracellular linker between S4 and S5 also results in a dramatic reduction of Mg(2+)-dependent modulation of Eag activation kinetics. Collectively, the two mutations in eag eliminate or even paradoxically reverse the effect of Mg(2+) on channel activation and inactivation kinetics. Together, these results suggest an important role of the intracellular linker regions in gating processes of Eag channels.
Identifier: FSU_pmch_20855938 (IID), 10.4161/chan.4.4.12329 (DOI), PMC3322480 (PMCID), 20855938 (RID), 20855938 (EID), 12329 (PII)
Grant Number: R01 NS050355, NS50355
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3322480.
Subject(s): Amino Acid Sequence
Animals
CHO Cells
Cricetinae
Cricetulus
Drosophila Proteins/chemistry
Drosophila Proteins/genetics
Drosophila Proteins/metabolism
Ether-A-Go-Go Potassium Channels/chemistry
Ether-A-Go-Go Potassium Channels/genetics
Ether-A-Go-Go Potassium Channels/metabolism
Genotype
Ion Channel Gating
Kinetics
Magnesium/metabolism
Membrane Potentials
Molecular Sequence Data
Mutagenesis, Site-Directed
Mutation
Patch-Clamp Techniques
Phenotype
Protein Conformation
Transfection
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_20855938
Owner Institution: FSU
Is Part Of: Channels (Austin, Tex.).
1933-6969
Issue: iss. 4, vol. 4

Choose the citation style.
Liu, X., Wu, Y., & Zhou, Y. (2010). Intracellular linkers are involved in Mg2+-dependent modulation of the Eag potassium channel. Channels (Austin, Tex.). Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_20855938