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Regulation of the transcription factor YY1 in mitosis through phosphorylation of its DNA-binding domain.

Title: Regulation of the transcription factor YY1 in mitosis through phosphorylation of its DNA-binding domain.
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Name(s): Rizkallah, Raed, author
Hurt, Myra M, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2009-11-01
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Yin-Yang 1 (YY1) is a ubiquitously expressed zinc finger transcription factor. It regulates a vast array of genes playing critical roles in development, differentiation, and cell cycle. Very little is known about the mechanisms that regulate the functions of YY1. It has long been proposed that YY1 is a phosphoprotein; however, a direct link between phosphorylation and the function of YY1 has never been proven. Investigation of the localization of YY1 during mitosis shows that it is distributed to the cytoplasm during prophase and remains excluded from DNA until early telophase. Immunostaining studies show that YY1 is distributed equally between daughter cells and rapidly associates with decondensing chromosomes in telophase, suggesting a role for YY1 in early marking of active and repressed genes. The exclusion of YY1 from DNA in prometaphase HeLa cells correlated with an increase in the phosphorylation of YY1 and loss of DNA-binding activity that can be reversed by dephosphorylation. We have mapped three phosphorylation sites on YY1 during mitosis and show that phosphorylation of two of these sites can abolish the DNA-binding activity of YY1. These results demonstrate a novel mechanism for the inactivation of YY1 through phosphorylation of its DNA-binding domain.
Identifier: FSU_pmch_19793915 (IID), 10.1091/mbc.E09-04-0264 (DOI), PMC2777106 (PMCID), 19793915 (RID), 19793915 (EID), E09-04-0264 (PII)
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2777106.
Subject(s): Amino Acid Sequence
Binding Sites
DNA/genetics
DNA/metabolism
Gene Expression Regulation
HeLa Cells/drug effects
HeLa Cells/physiology
Humans
Mitosis/drug effects
Mitosis/physiology
Mutagenesis, Site-Directed
Nocodazole/pharmacology
Phosphorylation
Serine/metabolism
Threonine/metabolism
Tubulin Modulators/pharmacology
YY1 Transcription Factor/genetics
YY1 Transcription Factor/metabolism
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_19793915
Owner Institution: FSU
Is Part Of: Molecular biology of the cell.
1939-4586
Issue: iss. 22, vol. 20

Choose the citation style.
Rizkallah, R., & Hurt, M. M. (2009). Regulation of the transcription factor YY1 in mitosis through phosphorylation of its DNA-binding domain. Molecular Biology Of The Cell. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_19793915