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molecular function of the yeast polo-like kinase Cdc5 in Cdc14 release during early anaphase.

Title: The molecular function of the yeast polo-like kinase Cdc5 in Cdc14 release during early anaphase.
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Name(s): Liang, Fengshan, author
Jin, Fengzhi, author
Liu, Hong, author
Wang, Yanchang, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2009-08-01
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: In the budding yeast Saccharomyces cerevisiae, Cdc14 is sequestered within the nucleolus before anaphase entry through its association with Net1/Cfi1, a nucleolar protein. Protein phosphatase PP2A(Cdc55) dephosphorylates Net1 and keeps it as a hypophosphorylated form before anaphase. Activation of the Cdc fourteen early anaphase release (FEAR) pathway after anaphase entry induces a brief Cdc14 release from the nucleolus. Some of the components in the FEAR pathway, including Esp1, Slk19, and Spo12, inactivate PP2A(Cdc55), allowing the phosphorylation of Net1 by mitotic cyclin-dependent kinase (Cdk) (Clb2-Cdk1). However, the function of another FEAR component, the Polo-like kinase Cdc5, remains elusive. Here, we show evidence indicating that Cdc5 promotes Cdc14 release primarily by stimulating the degradation of Swe1, the inhibitory kinase for mitotic Cdk. First, we found that deletion of SWE1 partially suppresses the FEAR defects in cdc5 mutants. In contrast, high levels of Swe1 impair FEAR activation. We also demonstrated that the accumulation of Swe1 in cdc5 mutants is responsible for the decreased Net1 phosphorylation. Therefore, we conclude that the down-regulation of Swe1 protein levels by Cdc5 promotes FEAR activation by relieving the inhibition on Clb2-Cdk1, the kinase for Net1 protein.
Identifier: FSU_pmch_19570916 (IID), 10.1091/mbc.E08-10-1049 (DOI), PMC2777927 (PMCID), 19570916 (RID), 19570916 (EID), E08-10-1049 (PII)
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2777927.
Subject(s): Anaphase/physiology
CDC2 Protein Kinase/genetics
CDC2 Protein Kinase/metabolism
Cell Cycle Proteins/genetics
Cell Cycle Proteins/metabolism
Cell Nucleolus/metabolism
Cyclin B/genetics
Cyclin B/metabolism
DNA, Ribosomal/metabolism
Nuclear Proteins/genetics
Nuclear Proteins/metabolism
Protein Kinases/genetics
Protein Kinases/metabolism
Protein Tyrosine Phosphatases/genetics
Protein Tyrosine Phosphatases/metabolism
Protein-Serine-Threonine Kinases
Protein-Tyrosine Kinases/genetics
Protein-Tyrosine Kinases/metabolism
Recombinant Fusion Proteins/genetics
Recombinant Fusion Proteins/metabolism
Saccharomyces cerevisiae/cytology
Saccharomyces cerevisiae/genetics
Saccharomyces cerevisiae/metabolism
Saccharomyces cerevisiae Proteins/genetics
Saccharomyces cerevisiae Proteins/metabolism
Signal Transduction/physiology
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_19570916
Owner Institution: FSU
Is Part Of: Molecular biology of the cell.
1939-4586
Issue: iss. 16, vol. 20

Choose the citation style.
Liang, F., Jin, F., Liu, H., & Wang, Y. (2009). The molecular function of the yeast polo-like kinase Cdc5 in Cdc14 release during early anaphase. Molecular Biology Of The Cell. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_19570916