You are here

Identification of G1-regulated genes in normally cycling human cells.

Title: Identification of G1-regulated genes in normally cycling human cells.
9 views
0 downloads
Name(s): Beyrouthy, Maroun J, author
Alexander, Karen E, author
Baldwin, Amy, author
Whitfield, Michael L, author
Bass, Hank W, author
McGee, Dan, author
Hurt, Myra M, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2008-01-01
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Obtaining synchronous cell populations is essential for cell-cycle studies. Methods such as serum withdrawal or use of drugs which block cells at specific points in the cell cycle alter cellular events upon re-entry into the cell cycle. Regulatory events occurring in early G1 phase of a new cell cycle could have been overlooked. We used a robotic mitotic shake-off apparatus to select cells in late mitosis for genome-wide gene expression studies. Two separate microarray experiments were conducted, one which involved isolation of RNA hourly for several hours from synchronous cell populations, and one experiment which examined gene activity every 15 minutes from late telophase of mitosis into G1 phase. To verify synchrony of the cell populations under study, we utilized methods including BrdU uptake, FACS, and microarray analyses of histone gene activity. We also examined stress response gene activity. Our analysis enabled identification of 200 early G1-regulated genes, many of which currently have unknown functions. We also confirmed the expression of a set of genes candidates (fos, atf3 and tceb) by qPCR to further validate the newly identified genes. Genome-scale expression analyses of the first two hours of G1 in naturally cycling cells enabled the discovery of a unique set of G1-regulated genes, many of which currently have unknown functions, in cells progressing normally through the cell division cycle. This group of genes may contain future targets for drug development and treatment of human disease.
Identifier: FSU_pmch_19079774 (IID), 10.1371/journal.pone.0003943 (DOI), PMC2600614 (PMCID), 19079774 (RID), 19079774 (EID)
Grant Number: R01 CA130795, R01 CA130795-01A2,
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2600614.
Subject(s): Animals
Bromodeoxyuridine/metabolism
CHO Cells
Cricetinae
Cricetulus
Down-Regulation/genetics
Flow Cytometry
G1 Phase/genetics
Gene Expression Profiling
Gene Expression Regulation
Genome, Human/genetics
HeLa Cells
Histones/genetics
Humans
Mitosis/genetics
Stress, Mechanical
Up-Regulation/genetics
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_19079774
Owner Institution: FSU
Is Part Of: PloS one.
1932-6203
Issue: iss. 12, vol. 3

Choose the citation style.
Beyrouthy, M. J., Alexander, K. E., Baldwin, A., Whitfield, M. L., Bass, H. W., McGee, D., & Hurt, M. M. (2008). Identification of G1-regulated genes in normally cycling human cells. Plos One. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_19079774