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Temporal control of the dephosphorylation of Cdk substrates by mitotic exit pathways in budding yeast.

Title: Temporal control of the dephosphorylation of Cdk substrates by mitotic exit pathways in budding yeast.
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Name(s): Jin, Fengzhi, author
Liu, Hong, author
Liang, Fengshan, author
Rizkallah, Raed, author
Hurt, Myra M, author
Wang, Yanchang, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2008-10-21
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: The temporal phosphorylation of cell cycle-related proteins by cyclin-dependent kinases (Cdks) is critical for the correct order of cell cycle events. In budding yeast, CDC28 encodes the only Cdk and its association with various cyclins governs the temporal phosphorylation of Cdk substrates. S-phase Cdk substrates are phosphorylated earlier than mitotic Cdk substrates, which ensures the sequential order of DNA synthesis and mitosis. However, it remains unclear whether Cdk substrates are dephosphorylated in temporally distinct windows. Cdc14 is a conserved protein phosphatase responsible for the dephosphorylation of Cdk substrates. In budding yeast, FEAR (Cdc14 early anaphase release) and MEN (mitotic exit network) activate phosphatase Cdc14 by promoting its release from the nucleolus in early and late anaphase, respectively. Here, we show that the sequential Cdc14 release and the distinct degradation timing of different cyclins provides the molecular basis for the differential dephosphorylation windows of S-phase and mitotic cyclin substrates. Our data also indicate that FEAR-induced dephosphorylation of S-phase Cdk substrates facilitates anaphase progression, revealing an extra layer of mitotic regulation.
Identifier: FSU_pmch_18845678 (IID), 10.1073/pnas.0808719105 (DOI), PMC2570984 (PMCID), 18845678 (RID), 18845678 (EID), 0808719105 (PII)
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2570984.
Subject(s): Cell Cycle Proteins/genetics
Cell Cycle Proteins/metabolism
Cyclin-Dependent Kinases/genetics
Cyclin-Dependent Kinases/metabolism
Cyclins/metabolism
Mitosis
Mutation/genetics
Phosphoprotein Phosphatases/genetics
Phosphoprotein Phosphatases/metabolism
Phosphorylation
Protein Tyrosine Phosphatases/genetics
Protein Tyrosine Phosphatases/metabolism
Saccharomycetales/cytology
Saccharomycetales/enzymology
Saccharomycetales/genetics
Substrate Specificity
Time Factors
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_18845678
Owner Institution: FSU
Is Part Of: Proceedings of the National Academy of Sciences of the United States of America.
1091-6490
Issue: iss. 42, vol. 105

Choose the citation style.
Jin, F., Liu, H., Liang, F., Rizkallah, R., Hurt, M. M., & Wang, Y. (2008). Temporal control of the dephosphorylation of Cdk substrates by mitotic exit pathways in budding yeast. Proceedings Of The National Academy Of Sciences Of The United States Of America. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_18845678