You are here

Pds1/Esp1-dependent and -independent sister chromatid separation in mutants defective for protein phosphatase 2A.

Title: Pds1/Esp1-dependent and -independent sister chromatid separation in mutants defective for protein phosphatase 2A.
Name(s): Tang, Xianying, author
Wang, Yanchang, author
Type of Resource: text
Genre: Journal Article
Date Issued: 2006-10-31
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Spindle disruption or DNA damage prevents sister chromatid separation through the activation of checkpoint pathways that inhibit anaphase entry by stabilizing the anaphase inhibitor Pds1. Mutation of CDC55, which encodes a B regulatory subunit of protein phosphatase 2A (PP2A), results in precocious sister chromatid separation when spindle is disrupted. Here we report that decreased Pds1 levels in Deltacdc55 mutants contribute to sister chromatid separation in the presence of nocodazole, a microtubule-depolymerizing drug. However, in the presence of DNA damage, Deltacdc55 mutant cells separate sister chromatids without noticeable decrease of Pds1 or cohesin Mcd1/Scc1 levels. Further analysis demonstrates that Deltacdc55 mutants lose cohesion along the entire chromosomes when the spindle is disrupted. In contrast, separation of sister chromatids is limited to the centromeric regions in Deltacdc55 cells after DNA damage. Moreover, mutation of TPD3, which encodes the A regulatory subunit of PP2A, also results in sister chromatid separation in DNA- or spindle-damage-arrested cells. These data suggest that PP2A regulates sister chromatid cohesion in Pds1-dependent and -independent manners.
Identifier: FSU_pmch_17050679 (IID), 10.1073/pnas.0607856103 (DOI), PMC1637575 (PMCID), 17050679 (RID), 17050679 (EID), 0607856103 (PII)
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at
Subject(s): Anaphase/genetics
Anaphase-Promoting Complex-Cyclosome
Cell Cycle Proteins/genetics
Cell Cycle Proteins/metabolism
Chromosome Segregation/genetics
DNA Damage/genetics
DNA, Fungal/genetics
Gene Deletion
Nuclear Proteins/genetics
Nuclear Proteins/metabolism
Phosphoprotein Phosphatases/genetics
Phosphoprotein Phosphatases/metabolism
Protein Phosphatase 2
Saccharomyces cerevisiae/drug effects
Saccharomyces cerevisiae/genetics
Saccharomyces cerevisiae/metabolism
Saccharomyces cerevisiae Proteins/genetics
Saccharomyces cerevisiae Proteins/metabolism
Spindle Apparatus/genetics
Ubiquitin-Protein Ligase Complexes/metabolism
Persistent Link to This Record:
Host Institution: FSU
Is Part Of: Proceedings of the National Academy of Sciences of the United States of America.
Issue: iss. 44, vol. 103

Choose the citation style.
Tang, X., & Wang, Y. (2006). Pds1/Esp1-dependent and -independent sister chromatid separation in mutants defective for protein phosphatase 2A. Proceedings Of The National Academy Of Sciences Of The United States Of America. Retrieved from