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function and regulation of budding yeast Swe1 in response to interrupted DNA synthesis.

Title: The function and regulation of budding yeast Swe1 in response to interrupted DNA synthesis.
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Name(s): Liu, Hong, author
Wang, Yanchang, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2006-06-01
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Periodically regulated cyclin-dependent kinase (Cdk) is required for DNA synthesis and mitosis. Hydroxyurea (HU) inhibits DNA synthesis by depleting dNTPs, the basic unit for DNA synthesis. HU treatment triggers the S-phase checkpoint, which arrests cells at S-phase, inhibits late origin firing and stabilizes replication forks. Using budding yeast as a model system, we found that Swe1, a negative regulator of Cdk, appears at S-phase and accumulates in HU treatment cells. Interestingly, this accumulation is not dependent on S-phase checkpoint. Deltahsl1, Deltahsl7, and cdc5-2 mutants, which have defects in Swe1 degradation, show HU sensitivity because of high Swe1 protein levels. We further demonstrated that their HU sensitivity is not a result of DNA damage accumulation or incomplete DNA synthesis; instead the sensitivity is due to their dramatically delayed recovery from HU-induced S-phase arrest. Strikingly, our in vivo data indicate that Swe1 inhibits the kinase activity of Clb2-Cdk1, but not that of Clb5-Cdk1. Therefore, S-phase accumulated Swe1 prevents Clb2-Cdk1-mediated mitotic activities, but has little effects on Clb5-Cdk1-associated S-phase progression.
Identifier: FSU_pmch_16571676 (IID), 10.1091/mbc.E05-11-1093 (DOI), PMC1474790 (PMCID), 16571676 (RID), 16571676 (EID), E05-11-1093 (PII)
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1474790.
Subject(s): CDC2 Protein Kinase/metabolism
Cell Cycle/drug effects
Cell Cycle/physiology
Cell Cycle Proteins/genetics
Cell Cycle Proteins/metabolism
Cyclin-Dependent Kinases/metabolism
Cyclins/metabolism
DNA Replication/drug effects
DNA Replication/physiology
Electrophoresis, Gel, Pulsed-Field
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Fungal
Hydroxyurea/pharmacology
Kinetics
Mitosis
Protein-Tyrosine Kinases/genetics
Protein-Tyrosine Kinases/metabolism
Saccharomyces cerevisiae Proteins/genetics
Saccharomyces cerevisiae Proteins/metabolism
Schizosaccharomyces/enzymology
Schizosaccharomyces/genetics
Schizosaccharomyces pombe Proteins/genetics
Schizosaccharomyces pombe Proteins/metabolism
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_16571676
Owner Institution: FSU
Is Part Of: Molecular biology of the cell.
1059-1524
Issue: iss. 6, vol. 17

Choose the citation style.
Liu, H., & Wang, Y. (2006). The function and regulation of budding yeast Swe1 in response to interrupted DNA synthesis. Molecular Biology Of The Cell. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_16571676