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Phosphatase 2A negatively regulates mitotic exit in Saccharomyces cerevisiae.

Title: Phosphatase 2A negatively regulates mitotic exit in Saccharomyces cerevisiae.
Name(s): Wang, Yanchang, author
Ng, Tuen-Yung, author
Type of Resource: text
Genre: Journal Article
Date Issued: 2006-01-01
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: In budding yeast Saccharomyces cerevisiae, Cdc5 kinase is a component of mitotic exit network (MEN), which inactivates cyclin-dependent kinase (CDK) after chromosome segregation. cdc5-1 mutants arrest at telophase at the nonpermissive temperature due to the failure of CDK inactivation. To identify more negative regulators of MEN, we carried out a genetic screen for genes that are toxic to cdc5-1 mutants when overexpressed. Genes that encode the B-regulatory subunit (Cdc55) and the three catalytic subunits (Pph21, Pph22, and Pph3) of phosphatase 2A (PP2A) were isolated. In addition to cdc5-1, overexpression of CDC55, PPH21, or PPH22 is also toxic to other temperature-sensitive mutants that display defects in mitotic exit. Consistently, deletion of CDC55 partially suppresses the temperature sensitivity of these mutants. Moreover, in the presence of spindle damage, PP2A mutants display nuclear localized Cdc14, the key player in MEN pathway, indicative of MEN activation. All the evidence suggests the negative role of PP2A in mitotic exit. Finally, our genetic and biochemical data suggest that PP2A regulates the phosphorylation of Tem1, which acts at the very top of MEN pathway.
Identifier: FSU_pmch_16079183 (IID), 10.1091/mbc.E04-12-1109 (DOI), PMC1345648 (PMCID), 16079183 (RID), 16079183 (EID), E04-12-1109 (PII)
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at
Subject(s): Cell Cycle Proteins/genetics
Cell Cycle Proteins/metabolism
Cytoskeletal Proteins/genetics
Cytoskeletal Proteins/metabolism
Gene Expression Regulation, Fungal/genetics
Genes, Lethal/genetics
Phosphoric Monoester Hydrolases/genetics
Phosphoric Monoester Hydrolases/metabolism
Protein Kinases/genetics
Protein Kinases/metabolism
Protein Phosphatase 2
Protein Tyrosine Phosphatases/genetics
Protein Tyrosine Phosphatases/metabolism
Protein-Serine-Threonine Kinases
Protein-Tyrosine Kinases/metabolism
Saccharomyces cerevisiae/cytology
Saccharomyces cerevisiae/enzymology
Saccharomyces cerevisiae/genetics
Saccharomyces cerevisiae Proteins/genetics
Saccharomyces cerevisiae Proteins/metabolism
Signal Transduction
Persistent Link to This Record:
Owner Institution: FSU
Is Part Of: Molecular biology of the cell.
Issue: iss. 1, vol. 17

Choose the citation style.
Wang, Y., & Ng, T. -Y. (2006). Phosphatase 2A negatively regulates mitotic exit in Saccharomyces cerevisiae. Molecular Biology Of The Cell. Retrieved from