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M(6)a Pathway Facilitates Sex Determination In Drosophila
| Title: | The M(6)a Pathway Facilitates Sex Determination In Drosophila. |
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| Name(s): |
Kan, Lijuan, author Grozhik, Anya V., author Vedanayagam, Jeffrey, author Patil, Deepak P., author Pang, Nan, author Lim, Kok-Seong, author Huang, Yi-Chun, author Joseph, Brian, author Lin, Ching-Jung, author Despic, Vladimir, author Guo, Jian, author Yan, Dong, author Kondo, Shu, author Deng, Wu-Min, author Dedon, Peter C., author Jaffrey, Samie R., author Lai, Eric C., author |
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| Type of Resource: | text | |
| Genre: |
Journal Article Text Journal Article |
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| Date Issued: | 2017-07-04 | |
| Physical Form: |
computer online resource |
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| Extent: | 1 online resource | |
| Language(s): | English | |
| Abstract/Description: | The conserved modification N-6-methyladenosine (m(6)A) modulates mRNA processing and activity. Here, we establish the Drosophila system to study the m(6)A pathway. We first apply miCLIP to map m(6)A across embryogenesis, characterize its m(6)A 'writer' complex, validate its YTH 'readers' CG6422 and YT521-B, and generate mutants in five m(6)A factors. While m(6)A factors with additional roles in splicing are lethal, m(6)A-specific mutants are viable but present certain developmental and behavioural defects. Notably, m(6)A facilitates the master female determinant Sxl, since multiple m(6)A components enhance female lethality in Sxl sensitized backgrounds. The m(6)A pathway regulates Sxl processing directly, since miCLIP data reveal Sxl as a major intronic m(6)A target, and female-specific Sxl splicing is compromised in multiple m(6)A pathway mutants. YT521-B is a dominant m(6)A effector for Sxl regulation, and YT521-B overexpression can induce female-specific Sxl splicing. Overall, our transcriptomic and genetic toolkit reveals in vivo biologic function for the Drosophila m(6)A pathway. | |
| Identifier: | FSU_libsubv1_wos_000404640800001 (IID), 10.1038/ncomms15737 (DOI) | |
| Keywords: | in-vivo, gene-expression, 3' utrs, clip data, fl(2)d gene, messenger-rna methylation, n-6-adenosine methylation, nuclear-rna, single-nucleotide-resolution, yth domain | |
| Publication Note: | The publisher's version of record is available at https://doi.org/10.1038/ncomms15737 | |
| Persistent Link to This Record: | http://purl.flvc.org/fsu/fd/FSU_libsubv1_wos_000404640800001 | |
| Owner Institution: | FSU | |
| Is Part Of: |
Nature Communications. 2041-1723 |
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| Issue: | vol. 8 |
Kan, L., Grozhik, A. V., Vedanayagam, J., Patil, D. P., Pang, N., Lim, K. -S., … Lai, E. C. (2017). The M(6)a Pathway Facilitates Sex Determination In Drosophila. Nature Communications. Retrieved from http://purl.flvc.org/fsu/fd/FSU_libsubv1_wos_000404640800001
