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M(6)a Pathway Facilitates Sex Determination In Drosophila

Title: The M(6)a Pathway Facilitates Sex Determination In Drosophila.
Name(s): Kan, Lijuan, author
Grozhik, Anya V., author
Vedanayagam, Jeffrey, author
Patil, Deepak P., author
Pang, Nan, author
Lim, Kok-Seong, author
Huang, Yi-Chun, author
Joseph, Brian, author
Lin, Ching-Jung, author
Despic, Vladimir, author
Guo, Jian, author
Yan, Dong, author
Kondo, Shu, author
Deng, Wu-Min, author
Dedon, Peter C., author
Jaffrey, Samie R., author
Lai, Eric C., author
Type of Resource: text
Genre: Journal Article
Journal Article
Date Issued: 2017-07-04
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: The conserved modification N-6-methyladenosine (m(6)A) modulates mRNA processing and activity. Here, we establish the Drosophila system to study the m(6)A pathway. We first apply miCLIP to map m(6)A across embryogenesis, characterize its m(6)A 'writer' complex, validate its YTH 'readers' CG6422 and YT521-B, and generate mutants in five m(6)A factors. While m(6)A factors with additional roles in splicing are lethal, m(6)A-specific mutants are viable but present certain developmental and behavioural defects. Notably, m(6)A facilitates the master female determinant Sxl, since multiple m(6)A components enhance female lethality in Sxl sensitized backgrounds. The m(6)A pathway regulates Sxl processing directly, since miCLIP data reveal Sxl as a major intronic m(6)A target, and female-specific Sxl splicing is compromised in multiple m(6)A pathway mutants. YT521-B is a dominant m(6)A effector for Sxl regulation, and YT521-B overexpression can induce female-specific Sxl splicing. Overall, our transcriptomic and genetic toolkit reveals in vivo biologic function for the Drosophila m(6)A pathway.
Identifier: FSU_libsubv1_wos_000404640800001 (IID), 10.1038/ncomms15737 (DOI)
Keywords: in-vivo, gene-expression, 3' utrs, clip data, fl(2)d gene, messenger-rna methylation, n-6-adenosine methylation, nuclear-rna, single-nucleotide-resolution, yth domain
Publication Note: The publisher's version of record is available at
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Owner Institution: FSU
Is Part Of: Nature Communications.
Issue: vol. 8

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Kan, L., Grozhik, A. V., Vedanayagam, J., Patil, D. P., Pang, N., Lim, K. -S., … Lai, E. C. (2017). The M(6)a Pathway Facilitates Sex Determination In Drosophila. Nature Communications. Retrieved from