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Mechanism Of Ribosome Rescue By Arfa And Rf2

Title: Mechanism Of Ribosome Rescue By Arfa And Rf2.
Name(s): Demo, Gabriel, author
Svidritskiy, Egor, author
Madireddy, Rohini, author
Diaz-Avalos, Ruben, author
Grant, Timothy, author
Grigorieff, Nikolaus, author
Sousa, Duncan, author
Korostelev, Andrei A., author
Type of Resource: text
Genre: Journal Article
Journal Article
Date Issued: 2017-03-16
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: ArfA rescues ribosomes stalled on truncated mRNAs by recruiting release factor RF2, which normally binds stop codons to catalyze peptide release. We report two 3.2 angstrom resolution cryo-EM structures - determined from a single sample - of the 70S ribosome with ArfA.RF2 in the A site. In both states, the ArfA C-terminus occupies the mRNA tunnel downstream of the A site. One state contains a compact inactive RF2 conformation. Ordering of the ArfA N-terminus in the second state rearranges RF2 into an extended conformation that docks the catalytic GGQ motif into the peptidyl-transferase center. Our work thus reveals the structural dynamics of ribosome rescue. The structures demonstrate how ArfA 'senses' the vacant mRNA tunnel and activates RF2 to mediate peptide release without a stop codon, allowing stalled ribosomes to be recycled.
Identifier: FSU_libsubv1_wos_000398146500001 (IID), 10.7554/eLife.23687 (DOI)
Keywords: messenger-rna, crystal-structure, structural basis, escherichia-coli, 70s ribosome, peptidyl-transfer-rna, release factor rf2, stalled ribosomes, stop codon, translation termination
Publication Note: The publisher's version of record is available at
Persistent Link to This Record:
Owner Institution: FSU
Is Part Of: Elife.
Issue: vol. 6

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Demo, G., Svidritskiy, E., Madireddy, R., Diaz-Avalos, R., Grant, T., Grigorieff, N., … Korostelev, A. A. (2017). Mechanism Of Ribosome Rescue By Arfa And Rf2. Elife. Retrieved from