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Dpb11 May Function With Rpa And Dna To Initiate Dna Replication

Title: Dpb11 May Function With Rpa And Dna To Initiate Dna Replication.
Name(s): Bruck, Irina, author
Dhingra, Nalini, author
Martinez, Matthew P., author
Kaplan, Daniel L., author
Type of Resource: text
Genre: Journal Article
Journal Article
Date Issued: 2017-05-03
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Dpb11 is required for the initiation of DNA replication in budding yeast. We found that Dpb11 binds tightly to single-stranded DNA (ssDNA) or branched DNA structures, while its human homolog, TopBP1, binds tightly to branched-DNA structures. We also found that Dpb11 binds stably to CDK-phosphorylated RPA, the eukaryotic ssDNA binding protein, in the presence of branched DNA. A Dpb11 mutant specifically defective for DNA binding did not exhibit tight binding to RPA in the presence of DNA, suggesting that Dpb11-interaction with DNA may promote the recruitment of RPA to melted DNA. We then characterized a mutant of Dpb11 that is specifically defective in DNA binding in budding yeast cells. Expression of dpb11-m1,2,3,5,Delta C results in a substantial decrease in RPA recruitment to origins, suggesting that Dpb11 interaction with DNA may be required for RPA recruitment to origins. Expression of dpb11-m1,2,3,5,Delta C also results in diminished GINS interaction with Mcm2-7 during S phase, while Cdc45 interaction with Mcm2-7 is like wild-type. The reduced GINS interaction with Mcm2-7 may be an indirect consequence of diminished origin melting. We propose that the tight interaction between Dpb11, CDK-phosphorylated RPA, and branched-DNA may be required for the essential function of stabilizing melted origin DNA in vivo. We also propose an alternative model, wherein Dpb11-DNA interaction is required for some other function in DNA replication initiation, such as helicase activation.
Identifier: FSU_libsubv1_wos_000400647000095 (IID), 10.1371/journal.pone.0177147 (DOI)
Keywords: saccharomyces-cerevisiae, protein, atp-hydrolysis, budding yeast, fork helicase, mcm2-7 helicase, origin dna, s-phase, single-stranded-dna, structural basis
Publication Note: The publisher's version of record is available at
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Owner Institution: FSU
Is Part Of: Plos One.
Issue: iss. 5, vol. 12

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Bruck, I., Dhingra, N., Martinez, M. P., & Kaplan, D. L. (2017). Dpb11 May Function With Rpa And Dna To Initiate Dna Replication. Plos One. Retrieved from