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Cleavage Of The Sun-domain Protein Mps3 At Its N-terminus Regulates Centrosome Disjunction In Budding Yeast Meiosis

Title: Cleavage Of The Sun-domain Protein Mps3 At Its N-terminus Regulates Centrosome Disjunction In Budding Yeast Meiosis.
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Name(s): Li, Ping, author
Jin, Hui, author
Koch, Bailey A., author
Abblett, Rebecca L., author
Han, Xuemei, author
Yates, John R., author
Yu, Hong-Guo, author
Type of Resource: text
Genre: Journal Article
Text
Journal Article
Date Issued: 2017-06
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Centrosomes organize microtubules and are essential for spindle formation and chromosome segregation during cell division. Duplicated centrosomes are physically linked, but how this linkage is dissolved remains unclear. Yeast centrosomes are tethered by a nuclear-envelope-attached structure called the half-bridge, whose components have mammalian homologues. We report here that cleavage of the half-bridge protein Mps3 promotes accurate centrosome disjunction in budding yeast. Mps3 is a single-pass SUN-domain protein anchored at the inner nuclear membrane and concentrated at the nuclear side of the half-bridge. Using the unique feature in yeast meiosis that centrosomes are linked for hours before their separation, we have revealed that Mps3 is cleaved at its nucleus-localized Nterminal domain, the process of which is regulated by its phosphorylation at serine 70. Cleavage of Mps3 takes place at the yeast centrosome and requires proteasome activity. We show that noncleavable Mps3 (Mps3-nc) inhibits centrosome separation during yeast meiosis. In addition, overexpression of mps3-nc in vegetative yeast cells also inhibits centrosome separation and is lethal. Our findings provide a genetic mechanism for the regulation of SUN-domain protein-mediated activities, including centrosome separation, by irreversible protein cleavage at the nuclear periphery.
Identifier: FSU_libsubv1_wos_000404512600017 (IID), 10.1371/journal.pgen.1006830 (DOI)
Keywords: mechanisms, binding, cell-cycle, chromosome segregation, duplication, kinase, nuclear-envelope, saccharomyces-cerevisiae, spindle pole body, tandem mass-spectrometry
Publication Note: The publisher's version of record is available at https://doi.org/10.1371/journal.pgen.1006830
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_libsubv1_wos_000404512600017
Owner Institution: FSU
Is Part Of: Plos Genetics.
1553-7404
Issue: iss. 6, vol. 13

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Li, P., Jin, H., Koch, B. A., Abblett, R. L., Han, X., Yates, J. R., & Yu, H. -G. (2017). Cleavage Of The Sun-domain Protein Mps3 At Its N-terminus Regulates Centrosome Disjunction In Budding Yeast Meiosis. Plos Genetics. Retrieved from http://purl.flvc.org/fsu/fd/FSU_libsubv1_wos_000404512600017