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Fin1-PP1 Helps Clear Spindle Assembly Checkpoint Protein Bub1 from Kinetochores in Anaphase

Title: Fin1-PP1 Helps Clear Spindle Assembly Checkpoint Protein Bub1 from Kinetochores in Anaphase.
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Name(s): Bokros, Michael, author
Gravenmier, Curtis, author
Jin, Fengzhi, author
Richmond, Daniel, author
Wang, Yanchang, author
Type of Resource: text
Genre: Text
Date Issued: 2016-02-09
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: The spindle assembly checkpoint (SAC) monitors chromosome attachment defects, and the assembly of SAC proteins at kinetochores is essential for its activation, but the SAC disassembly process remains unknown. We found that deletion of a 14-3-3 protein, Bmh1, or hyperactivation of Cdc14 early anaphase release (FEAR) allows premature SAC silencing in budding yeast, which depends on a kinetochore protein Fin1 that forms a complex with protein phosphatase PP1. Previous works suggest that FEAR-dependent Fin1 dephosphorylation promotes Bmh1-Fin1 dissociation, which enables kinetochore recruitment of Fin1-PP1. We found persistent kinetochore association of SAC protein Bub1 in fin1 Delta mutants after anaphase entry. Therefore, we revealed a mechanism that clears SAC proteins from kinetochores. After anaphase entry, FEAR activation promotes kinetochore enrichment of Fin1-PP1, resulting in SAC disassembly at kinetochores. This mechanism is required for efficient SAC silencing after SAC is challenged, and untimely Fin1-kinetochore association causes premature SAC silencing and chromosome missegregation.
Identifier: FSU_libsubv1_wos_000369616100011 (IID), 10.1016/j.celrep.2016.01.007 (DOI)
Keywords: attachment, budding yeast, cdc14, dna-damage, kinase, mitotic exit, phosphatase 2a, phosphorylation, separase, yeast saccharomyces-cerevisiae
Publication Note: The publisher’s version of record is available at http://www.dx.doi.org/10.1016/j.celrep.2016.01.007
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_libsubv1_wos_000369616100011
Owner Institution: FSU
Is Part Of: Cell Reports.
2211-1247
Issue: iss. 5, vol. 14

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Bokros, M., Gravenmier, C., Jin, F., Richmond, D., & Wang, Y. (2016). Fin1-PP1 Helps Clear Spindle Assembly Checkpoint Protein Bub1 from Kinetochores in Anaphase. Cell Reports. Retrieved from http://purl.flvc.org/fsu/fd/FSU_libsubv1_wos_000369616100011