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Hierarchical regulation of the genome

Title: Hierarchical regulation of the genome: global changes in nucleosome organization potentiate genome response.
Name(s): Sexton, Brittany S., author
Druliner, Brooke R., author
Vera, Daniel L., author
Avey, Denis, author
Zhu, Fanxiu, author
Dennis, Jonathan H., author
Type of Resource: text
Genre: Text
Date Issued: 2016-02-09
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Nucleosome occupancy is critically important in regulating access to the eukaryotic genome. Few studies in human cells have measured genome-wide nucleosome distributions at high temporal resolution during a response to a common stimulus. We measured nucleosome distributions at high temporal resolution following Kaposi's-sarcoma-associated herpesvirus (KSHV) reactivation using our newly developed mTSS-seq technology, which maps nucleosome distribution at the transcription start sites (TSS) of all human genes. Nucleosomes underwent widespread changes in organization 24 hours after KSHV reactivation and returned to their basal nucleosomal architecture 48 hours after KSHV reactivation. The widespread changes consisted of an indiscriminate remodeling event resulting in the loss of nucleosome rotational phasing signals. Additionally, one in six TSSs in the human genome possessed nucleosomes that are translationally remodeled. 72% of the loci with translationally remodeled nucleosomes have nucleosomes that moved to positions encoded by the underlying DNA sequence. Finally we demonstrated that these widespread alterations in nucleosomal architecture potentiated regulatory factor binding. These descriptions of nucleosomal architecture changes provide a new framework for understanding the role of chromatin in the genomic response, and have allowed us to propose a hierarchical model for chromatin-based regulation of genome response.
Identifier: FSU_libsubv1_wos_000376123100009 (IID), 10.18632/oncotarget.6841 (DOI)
Keywords: cell-line, chromatin, Chromosome Section, dna-sequences, endothelial origin, gene-expression, iSLK.219, kaposis-sarcoma, KSHV, next generation sequencing, nucleosome, particle, promoter, resolution, sarcoma-associated herpesvirus, transcription factors
Publication Note: The publisher’s version of record is available at
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Owner Institution: FSU
Is Part Of: Oncotarget.
Issue: iss. 6, vol. 7

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Sexton, B. S., Druliner, B. R., Vera, D. L., Avey, D., Zhu, F., & Dennis, J. H. (2016). Hierarchical regulation of the genome: global changes in nucleosome organization potentiate genome response. Oncotarget. Retrieved from