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Comprehensive nucleosome mapping of the human genome in cancer progression

Title: Comprehensive Nucleosome Mapping Of The Human Genome In Cancer Progression.
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Name(s): Druliner, Brooke R., author
Vera, Daniel, author
Johnson, Ruth, author
Ruan, Xiaoyang, author
Apone, Lynn M., author
Dimalanta, Eileen T., author
Stewart, Fiona J., author
Boardman, Lisa, author
Dennis, Jonathan H., author
Type of Resource: text
Genre: Text
Date Issued: 2016-03-22
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Altered chromatin structure is a hallmark of cancer, and inappropriate regulation of chromatin structure may represent the origin of transformation. Important studies have mapped human nucleosome distributions genome wide, but the role of chromatin structure in cancer progression has not been addressed. We developed a MNase-Transcription Start Site Sequence Capture method (mTSS-seq) to map the nucleosome distribution at human transcription start sites genome-wide in primary human lung and colon adenocarcinoma tissue. Here, we confirm that nucleosome redistribution is an early, widespread event in lung (LAC) and colon (CRC) adenocarcinoma. These altered nucleosome architectures are consistent between LAC and CRC patient samples indicating that they may serve as important early adenocarcinoma markers. We demonstrate that the nucleosome alterations are driven by the underlying DNA sequence and potentiate transcription factor binding. We conclude that DNA-directed nucleosome redistributions are widespread early in cancer progression. We have proposed an entirely new hierarchical model for chromatin-mediated genome regulation.
Identifier: FSU_libsubv1_wos_000375687200013 (IID), 10.1101/021618 (DOI)
Keywords: cancer, chromatin, Chromosome Section, dna, flexibility, genes, lung adenocarcinoma, MNase, nucleosome, organization, resolution, sequence, somatic mutations, susceptibility, whole genome
Publication Note: The publisher’s version of record is available at https://doi.org/10.1101/021618
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_libsubv1_wos_000375687200013
Owner Institution: FSU
Is Part Of: Oncotarget.
1949-2553
Issue: iss. 12, vol. 7

Choose the citation style.
Druliner, B. R., Vera, D., Johnson, R., Ruan, X., Apone, L. M., Dimalanta, E. T., … Dennis, J. H. (2016). Comprehensive Nucleosome Mapping Of The Human Genome In Cancer Progression. Oncotarget. Retrieved from http://purl.flvc.org/fsu/fd/FSU_libsubv1_wos_000375687200013