You are here

An improved smaller biotin ligase for BioID proximity labeling

Title: An improved smaller biotin ligase for BioID proximity labeling.
254 views
195 downloads
Name(s): Kim, Dae In, author
Jensen, Samuel C., author
Noble, Kyle A., author
Birendra, K. C., author
Roux, Kenneth H., author
Motamedchaboki, Khatereh, author
Roux, Kyle J., author
Type of Resource: text
Genre: Text
Date Issued: 2016-04-15
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: The BioID method uses a promiscuous biotin ligase to detect protein-protein associations as well as proximate proteins in living cells. Here we report improvements to the BioID method centered on BioID2, a substantially smaller promiscuous biotin ligase. BioID2 enables more-selective targeting of fusion proteins, requires less biotin supplementation, and exhibits enhanced labeling of proximate proteins. Thus BioID2 improves the efficiency of screening for protein-protein associations. We also demonstrate that the biotinylation range of BioID2 can be considerably modulated using flexible linkers, thus enabling application-specific adjustment of the biotin-labeling radius.
Identifier: FSU_libsubv1_wos_000375753600003 (IID), 10.1091/mbc.E15-12-0844 (DOI)
Keywords: dependent biotinylation, e-cadherin, interacting partners, mammalian-cells, membrane, messenger-rna export, nuclear-pore complex, promiscuous protein biotinylation, proteomic analysis, trypanosoma-brucei
Publication Note: The publisher’s version of record is available at http://www.dx.doi.org/10.1091/mbc.E15-12-0844
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_libsubv1_wos_000375753600003
Owner Institution: FSU
Is Part Of: Molecular Biology of the Cell.
1059-1524
Issue: iss. 8, vol. 27

Choose the citation style.
Kim, D. I., Jensen, S. C., Noble, K. A., Birendra, K. C., Roux, K. H., Motamedchaboki, K., & Roux, K. J. (2016). An improved smaller biotin ligase for BioID proximity labeling. Molecular Biology Of The Cell. Retrieved from http://purl.flvc.org/fsu/fd/FSU_libsubv1_wos_000375753600003