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Biochemical Mechanisms for Geographical Adaptations to Novel Toxin Exposures in Butterflyfish

Title: Biochemical Mechanisms for Geographical Adaptations to Novel Toxin Exposures in Butterflyfish.
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Name(s): Maldonado, Aileen, author
Lavado, Ramon, author
Knuston, Sean, author
Slattery, Marc, author
Ankisetty, Sridevi, author
Goldstone, Jared V., author
Watanabe, Kayo, author
Hoh, Eunha, author
Gadepalli, Rama S., author
Rimoldi, John M., author
Ostrander, Gary K., author
Schlenk, Daniel, author
Type of Resource: text
Genre: Text
Date Issued: 2016-05-03
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Some species of butterflyfish have had preyed upon corals for millions of years, yet the mechanism of butterflyfish specialized coral feeding strategy remains poorly understood. Certain butterflyfish have the ability to feed on allelochemically rich soft corals, e.g. Sinularia maxima. Cytochrome P450 (CYP) is the predominant enzyme system responsible for the detoxification of dietary allelochemicals. CYP2-like and CYP3A-like content have been associated with butterflyfish that preferentially consumes allelochemically rich soft corals. To investigate the role of butterflyfish CYP2 and CYP3A enzymes in dietary preference, we conducted oral feeding experiments using homogenates of S. maxima and a toxin isolated from the coral in four species of butterflyfish with different feeding strategies. After oral exposure to the S. maxima toxin 5-episinulaptolide (5ESL), which is not normally encountered in the Hawaiian butterflyfish diet, an endemic specialist, Chaetodon multicinctus experienced 100% mortality compared to a generalist, Chaetodon auriga, which had significantly more (3-6 fold higher) CYP3A-like basal content and catalytic activity. The specialist, Chaetodon unimaculatus, which preferentially feed on S. maxima in Guam, but not in Hawaii, had 100% survival, a significant induction of 8-12 fold CYP3A-like content, and an increased ability (2-fold) to metabolize 5ESL over other species. Computer modeling data of CYP3A4 with 5ESL were consistent with microsomal transformation of 5ESL to a C15-16 epoxide from livers of C. unimaculatus. Epoxide formation correlated with CYP3A-like content, catalytic activity, induction, and NADPH-dependent metabolism of 5ESL. These results suggest a potentially important role for the CYP3A family in butterflyfish-coral diet selection through allelochemical detoxification.
Identifier: FSU_libsubv1_wos_000375675700020 (IID), 10.1371/journal.pone.0154208 (DOI)
Keywords: Chaetodon, chemical defenses, coral-feeding fishes, cytochrome-p450, dietary specialization, expression, generalist fish predators, molecular-cloning, plant secondary compounds, reef
Publication Note: The publisher’s version of record is available at http://www.dx.doi.org/10.1371/journal.pone.0154208
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_libsubv1_wos_000375675700020
Owner Institution: FSU
Is Part Of: Plos One.
1932-6203
Issue: iss. 5, vol. 11

Choose the citation style.
Maldonado, A., Lavado, R., Knuston, S., Slattery, M., Ankisetty, S., Goldstone, J. V., … Schlenk, D. (2016). Biochemical Mechanisms for Geographical Adaptations to Novel Toxin Exposures in Butterflyfish. Plos One. Retrieved from http://purl.flvc.org/fsu/fd/FSU_libsubv1_wos_000375675700020