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The DEAD-box Protein Rok1 Orchestrates 40S and 60S Ribosome Assembly by Promoting the Release of Rrp5 from Pre-40S Ribosomes to Allow for 60S Maturation

Title: The Dead-box Protein Rok1 Orchestrates 40s And 60s Ribosome Assembly By Promoting The Release Of Rrp5 From Pre-40s Ribosomes To Allow For 60s Maturation.
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Name(s): Khoshnevis, Sohail, author
Askenasy, Isabel, author
Johnson, Matthew C., author
Dattolo, Maria D., author
Young-Erdos, Crystal L., author
Stroupe, M. Elizabeth, author
Karbstein, Katrin, author
Type of Resource: text
Genre: Text
Date Issued: 2016-06
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: DEAD-box proteins are ubiquitous regulators of RNA biology. While commonly dubbed "helicases," their activities also include duplex annealing, adenosine triphosphate (ATP)-dependent RNA binding, and RNA-protein complex remodeling. Rok1, an essential DEAD-box protein, and its cofactor Rrp5 are required for ribosome assembly. Here, we use in vivo and in vitro biochemical analyses to demonstrate that ATP-bound Rok1, but not adenosine diphosphate (ADP)-bound Rok1, stabilizes Rrp5 binding to 40S ribosomes. Interconversion between these two forms by ATP hydrolysis is required for release of Rrp5 from pre-40S ribosomes in vivo, thereby allowing Rrp5 to carry out its role in 60S subunit assembly. Furthermore, our data also strongly suggest that the previously described accumulation of snR30 upon Rok1 inactivation arises because Rrp5 release is blocked and implicate a previously undescribed interaction between Rrp5 and the DEAD-box protein Has1 in mediating snR30 accumulation when Rrp5 release from pre-40S subunits is blocked.
Identifier: FSU_libsubv1_wos_000378611200007 (IID), 10.1371/journal.pbio.1002480 (DOI)
Keywords: cryo-em, electron-microscopy images, helicase, junction core complex, pre-ribosomes, RNA, saccharomyces-cerevisiae, subunit biogenesis, translation initiation, yeast
Publication Note: The publisher’s version of record is available at https://doi.org/10.1371/journal.pbio.1002480
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_libsubv1_wos_000378611200007
Owner Institution: FSU
Is Part Of: Plos Biology.
1545-7885
Issue: iss. 6, vol. 14

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Khoshnevis, S., Askenasy, I., Johnson, M. C., Dattolo, M. D., Young-Erdos, C. L., Stroupe, M. E., & Karbstein, K. (2016). The Dead-box Protein Rok1 Orchestrates 40s And 60s Ribosome Assembly By Promoting The Release Of Rrp5 From Pre-40s Ribosomes To Allow For 60s Maturation. Plos Biology. Retrieved from http://purl.flvc.org/fsu/fd/FSU_libsubv1_wos_000378611200007