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Examining the Role of EcR Isoforms in the Regulation of Cut at the Dorsal-Ventral Boundary of the Drosophila Imaginal Wing Disc

Title: Examining the Role of EcR Isoforms in the Regulation of Cut at the Dorsal-Ventral Boundary of the Drosophila Imaginal Wing Disc.
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Name(s): Bryant, Jamal M., author
Jia, Dongyu, author
Deng, Wu-Min, author
Department of Chemistry and Biochemistry
Type of Resource: text
Genre: Text
Issuance: monographic
Date Issued: 2015
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: The precise regulation of cellular and hormonal signaling pathways is required for proper development in metazoan organisms. The Drosophila imaginal wing disc is the ideal model system for the study of cellular signaling regulating growth and development. The canonical Notch signaling pathway is known to control the formation of the dorsal-ventral boundary in the wing disc through the regulation of Cut. Our recent findings suggest Ecdysone signaling regulates Cut in the DV-boundary as well. The Ecdysone receptor is a heterodimer of USP and EcR. EcR has three protein isomers: EcR-A, EcR-B1, and EcR-B2. Because EcR-B1 is the most prevalent isoform of the Ecdysone receptor, it is the most widely used in loss of function studies of Ecdysone signaling. Here, we examine the EcR-A and EcR-B2 isoforms to determine the effect their loss of function has on the expression of Cut at the DV boundary, as well as on Notch downstream targets. We report that, in addition to the EcR-B1 receptor, Ecdysone signaling also regulates Cut via the EcR-B2 receptor isoform, but not the EcR-A isoform. We also found that each of the three EcR isoforms can suppress aberrant high Notch activation at the DV boundary, and that the EcR-B1 and EcR-B2 isoforms regulate the Notch target Wg as well.
Identifier: FSU_migr_uhm-0479 (IID)
Keywords: Ecdysone, Notch, Drosophila, Wing disc, cut, EcR
Submitted Note: A Thesis submitted to the Department of Chemistry and Biochemistry in partial fulfillment of the requirements for graduation with Honors in the Major.
Degree Awarded: Spring Semester, 2015.
Date of Defense: April 23, 2015.
Subject(s): Biology
Developmental biology
Genetics
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_migr_uhm-0479
Owner Institution: FSU
Is Part of Series: Honors Theses.

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Bryant, J. M., Jia, D., & Deng, W. -M. (2015). Examining the Role of EcR Isoforms in the Regulation of Cut at the Dorsal-Ventral Boundary of the Drosophila Imaginal Wing Disc. Retrieved from http://purl.flvc.org/fsu/fd/FSU_migr_uhm-0479