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Φ-Value Analysis of Symfoil-4T

Title: Φ-Value Analysis of Symfoil-4T.
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Name(s): Sutherland, Mason A., author
Department of Biological Science
Type of Resource: text
Genre: Text
Issuance: monographic
Date Issued: 2015
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: A critical consideration in the process of de novo protein architecture design and protein evolution is the folding pathway and behavior a protein undertakes in transitioning to its functional tertiary structure. Of particular interest is a cryptic element within protein primary structure that enables an efficient folding pathway, and is postulated to be a heritable element in the evolution of protein architecture, the "folding nucleus" (FN). However, almost nothing is known regarding how the FN changes as simpler peptide motifs join to form more complex polypeptides. To this effect, the structure and folding properties of foldable intermediates along the evolutionary trajectory of the β-trefoil protein type were tested. This study specifically used and compared data from Symfoil-4T (an engineered β-trefoil protein) to several mutants to show that the FN is acquired during gene fusion events, incorporating novel turn structure generated by gene fusion. Furthermore, the FN of β-trefoils are adjusted by circular permutation in response to destabilizing functional mutations to allow the survival of FN (which is made possible by the intrinsic C3 cyclic symmetry of β-trefoil architecture) identifying a selective advantage that helps explain extant cyclic structural symmetry in the proteome.
Identifier: FSU_migr_uhm-0453 (IID)
Keywords: protein, folding, FGF-1, Symfoil-4T
Submitted Note: A Thesis submitted to the Department of Biological Sciences in partial fulfillment of the requirements for graduation with Honors in the Major.
Degree Awarded: Spring Semester, 2017.
Date of Defense: December 1, 2015.
Subject(s): Amino acids
Peptides
Proteins
Biochemistry
Biophysics
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_migr_uhm-0453
Owner Institution: FSU
Is Part of Series: Honors Theses.

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Sutherland, M. A. (2015). Φ-Value Analysis of Symfoil-4T. Retrieved from http://purl.flvc.org/fsu/fd/FSU_migr_uhm-0453