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Enzymatic Hydrolysis Kinetics

Title: Enzymatic Hydrolysis Kinetics.
Name(s): Tacey, Sean, author
Department of Chemical Engineering
Type of Resource: text
Genre: Text
Issuance: serial
Date Issued: 2013
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Energy has become a much needed commodity throughout the world; as such, the development of environmentally safe fuel sources is of great importance. Agricultural residues (cellulosic material) are turning out be an attractive source for production of biofuels due to their cost, availability and power to generate required quantities of fuel. A method to convert these residues to fuel is by enzymatically breaking them down to form sugars and then fermenting these sugars to produce useful fuels and chemicals. However, significant challenges remain in developing an economic and efficient conversion process. One challenge is in determining the rate at which enzymes break down the cellulosic material and in developing a comprehensive model which aids in the design of reactors for biofuel production. My work has focused on determining the rates at which the enzymatic hydrolysis of cellulose into glucose occurs, and how outside factors can alter these rates. This has been accomplished through the use of a quartz crystal microbalance (QCM), a device that detects minute changes in the mass (i.e. nanogram quantities) of a coated material through frequency measurements. The gold crystals used in the QCM device are coated with a thin cellulose film using an environmentally friendly solvent, NMMO. Cellulose hydrolysis has been studied using commercial enzyme mixtures and it has been discerned that the QCM can directly measure the amount of enzyme adsorbed, as well as the subsequent rates of hydrolysis, in a single measurement – factors which will aid in the development of a theoretical model. Further, efforts have been made to study the effect of different products (e.g. glucose and solvent) in regard to their influence on the rates of the hydrolysis reaction. This will aid in developing a comprehensive model of the hydrolysis process.
Identifier: FSU_migr_undergradresearch-0001 (IID)
Subject(s): Chemical engineering
Persistent Link to This Record:
Owner Institution: FSU
Is Part of Series: Showcase of Undergraduate Research Excellence.

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Tacey, S. (2013). Enzymatic Hydrolysis Kinetics. Retrieved from