You are here

Myofilament Calcium De-Sensitization and Contractile Uncoupling Prevent Pause-Triggered Ventricular Tachycardia in Mouse Hearts with Chronic Myocardial Infarction

Title: Myofilament Calcium De-Sensitization and Contractile Uncoupling Prevent Pause-Triggered Ventricular Tachycardia in Mouse Hearts with Chronic Myocardial Infarction.
177 views
41 downloads
Name(s): Venkataraman, Raghav, 1984-, author
Baldo, Marcelo, author
Hwang, Hyun, author
Veltri, Tiago, author
Pinto, Jose, author
Baudenbacher, Franz, author
Knollmann, Björn C., author
Type of Resource: text
Genre: Text
Issuance: serial
Date Issued: 2013
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Myocardial infarction (MI) is a major risk for ventricular arrhythmia. Pause-triggered ventricular arrhythmia can be caused by increased myofilament Ca binding due to sarcomeric mutations or Ca-sensitizing compounds. Myofilament Ca sensitivity is also increased after MI. Here we hypothesize that MI increases risk for pause-triggered ventricular arrhythmias, which can be prevented by myofilament Ca-desensitization and contractile uncoupling. To test this hypothesis, we generated a murine chronic MI model using male B6SJLF1/J mice (n=40) that underwent permanent ligation of the left anterior descending coronary artery. 4 weeks post MI, cardiac structure, function and myofilament Ca sensitivity were evaluated. Pause-dependent arrhythmia susceptibility was quantified in isolated hearts with pacing trains of increasing frequency, followed by a pause and an extra stimulus. Coronary ligation resulted in a mean infarct size of 39.6±5.7% LV and fractional shortening on echocardiography was reduced by 40% compared to non-infarcted controls. Myofilament Ca sensitivity was significantly increased in post MI hearts (pCa50: Control=5.66±0.03; MI=5.84±0.05; P
Identifier: FSU_migr_biomed_faculty_publications-0057 (IID), 10.1016/j.yjmcc.2013.03.022 (DOI)
Keywords: blebbistatin, chronic MI, ventricular tachycardia, mouse hearts, myofilament Ca sensitivity, triggered arrhythmia
Uncontrolled subjects: Animals, Calcium, Chronic Disease, Disease Models, Animal, Heterocyclic Compounds with 4 or More Rings, Male, Mice, Myocardial Contraction, Myocardial Infarction, Myocardium, Myofibrils, Tachycardia, Ventricular
Note: Originally published in Journal'>http://www.sciencedirect.com/science/article/pii/S002228281300103X">Journal of Molecular and Cellular Cardiology.
Citation: Venkataraman R1, Baldo MP, Hwang HS, Veltri T, Pinto JR, Baudenbacher FJ, & Knollmann BC. (2013). Myofilament calcium de-sensitization and contractile uncoupling prevent pause-triggered ventricular tachycardia in mouse hearts with chronic myocardial infarction. J Mol Cell Cardiol, Jul;60:8-15. doi: 10.1016/j.yjmcc.2013.03.022.
Subject(s): Cardiology
Cardiovascular system -- Diseases
Cardiovascular system
Heterocyclic compounds
Inorganic compounds
Investigations
Medical sciences
Pathology
Symptoms
Links: http://dx.doi.org/10.1016/j.yjmcc.2013.03.022
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_migr_biomed_faculty_publications-0057
Owner Institution: FSU
Is Part of Series: Department of Biomedical Sciences Faculty Publications.
Is Part Of: Journal of Molecular and Cellular Cardiology.
Issue: 60

Choose the citation style.
Venkataraman, R., Baldo, M., Hwang, H., Veltri, T., Pinto, J., Baudenbacher, F., & Knollmann, B. C. (2013). Myofilament Calcium De-Sensitization and Contractile Uncoupling Prevent Pause-Triggered Ventricular Tachycardia in Mouse Hearts with Chronic Myocardial Infarction. Journal Of Molecular And Cellular Cardiology. Retrieved from http://dx.doi.org/10.1016/j.yjmcc.2013.03.022