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Development of Microcontact Printing Techniques for Fabricating Functional Micropatterns and Microparticles for Biomedical Applications

Title: Development of Microcontact Printing Techniques for Fabricating Functional Micropatterns and Microparticles for Biomedical Applications.
Name(s): Wang, Zhibin, author
Guan, Jingjiao, 1973-, professor directing dissertation
Liu, Tao, 1969-, university representative
Lenhert, Steven, committee member
Siegrist, Theo M., committee member
Paravastu, Anant K., committee member
Florida State University, degree granting institution
College of Engineering, degree granting college
Department of Chemical and Biomedical Engineering, degree granting department
Type of Resource: text
Genre: Text
Issuance: monographic
Date Issued: 2015
Publisher: Florida State University
Place of Publication: Tallahassee, Florida
Physical Form: computer
online resource
Extent: 1 online resource (110 pages)
Language(s): English
Abstract/Description: Microcontact printing (μCP) is a soft lithographic technique based on transferring an ink material from a stamp on to a substrate through physical contact to produce features at the micrometer scale. Compared to other surface-patterning techniques, μCP is known for ease of use, low cost, high reliability, and high versatility. It is being used across diverse fields of scientific and engineering research, and holds potential to be useful for large-scale manufacturing. My doctoral research is focused on the development of μCP techniques for fabricating functional micropatterns and microparticles for biomedical applications. Polyelectrolyte Specifically, my work consists of the following four projects. First, I developed a method based on μCP of polyelectrolyte for surface micropatternng. The method is featured by the use of an unmodified poly(dimethyl siloxane) (PDMS) stamp to print polyelectrolytes on a substrate coated with a monolayer of poly(ethylene glycol) (PEG)-silane. The method is applicable to various polyelectrolytes including poly(allylamine hydrochloride) (PAH), branched and linear poly(ethylene imine) (PEI), poly-L-lysine (PLL), poly(diallyldimethylammonium chloride) (PDAC), chitosan, double stranded DNA, and poly(sodium 4-styrene sulfonate) (PSS). The printed polyelectrolyte structures, which include monolayer, bilayer, and stretched molecular bundles, are stable in aqueous solutions and have been used for micropatterning quantum dot nanoparticles, DNA, proteins, and live cells. Second, I utilized a novel polyelectrolyte ink material for μCP. The ink material is poly(4-aminostyrene) (PAS) which can be converted to aryldiazonium salt and exhibits pH-dependent hydrophobicity. I demonstrated that PAS could be microcontact printed using an unmodified PDMS stamp, and the printed PAS could be diazotized to further micropattern biomolecules including DNA and protein and nanomaterials including single-walled carbon nanotubes and gold nanoparticles. I also revealed that the diazotized PAS could be used to enable μCP of a metallic structure on a carbon surface. Third, the hydrophobic nature of PAS at the neutral pH allowed the microcontact-printed PAS-based polyelectrolyte multilayer to be used as masks for wet etching. In addition, I used this technique to produce highly engineered microparticles. Third, while investigating the diazonium chemistry of PAS, I developed a novel paper indicator for sensing nitrite The paper indicator is featured by using three different modalities including colorimetric assay, Raman spectroscopy, and electron paramagnetic resonance spectroscopy. This method is simple and inexpensive, and promises to have wider applicability than the existing paper indicators for nitrite sensing. Lastly, I developed a novel μCP technique for functionalizing and assembling live cells by integrating μCP of polymeric biomaterials with the use of a temperature-sensitive water-soluble sacrificial layer. It can not only functionalize live cells with microscopic polyelectrolyte and thermoplastic structures of various sizes and shapes, but also to assemble the cells into macroscopic stripes and sheets. The technique is also applicable to multiple types of cells, including human leukemic cells, mouse embryonic stem cells and human mesenchymal stem cells in the forms of single cells and cell aggregates. In addition, the technique can be used with biodegradable and biocompatible polyelectrolytes and thermoplastic. Compared with the conventional methods, this technique is inexpensive, easy to use, highly versatile and potentially useful for diverse biomedical applications, such as drug delivery, cellular therapies and tissue engineering.
Identifier: FSU_migr_etd-9519 (IID)
Submitted Note: A Dissertation submitted to the Department of Chemical and Biomedical Engineering in partial fulfillment of the requirements for the degree of Doctor of Philosophy.
Degree Awarded: Summer Semester 2015.
Date of Defense: May 12, 2015.
Keywords: Layer-by-layer, Microcontact Printing, Microparticles, Micropatterns, Polyelectrolyte, Self-Assembled Monolayers
Bibliography Note: Includes bibliographical references.
Advisory Committee: Jingjiao Guan, Professor Directing Dissertation; Tao Liu, University Representative; Steven Lenhert, Committee Member; Theo M. Siegrist, Committee Member; Anant Paravastu, Committee Member.
Subject(s): Chemical engineering
Biomedical engineering
Materials science
Persistent Link to This Record:
Owner Institution: FSU

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Wang, Z. (2015). Development of Microcontact Printing Techniques for Fabricating Functional Micropatterns and Microparticles for Biomedical Applications. Retrieved from