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Cryopreservation of Pluripotent Stem Cell-Derived Neural Progenitor Aggregates Labelled with Micron-Sized Particles of Iron Oxide

Title: Cryopreservation of Pluripotent Stem Cell-Derived Neural Progenitor Aggregates Labelled with Micron-Sized Particles of Iron Oxide.
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Name(s): Yan, Yuanwei, author
Li, Yan, professor directing thesis
Grant, Samuel C., committee member
Guan, Jingjiao, 1973-, committee member
Florida State University, degree granting institution
College of Engineering, degree granting college
Type of Resource: text
Genre: Text
Issuance: monographic
Date Issued: 2014
Publisher: Florida State University
Place of Publication: Tallahassee, Florida
Physical Form: computer
online resource
Extent: 1 online resource (80 pages)
Language(s): English
Abstract/Description: Magnetic resonance imaging (MRI) provides an effective approach to track the labeled pluripotent stem cell (PSC)-derived neural progenitor cells (NPCs) for neural transplantation and neurological disorder treatments. However, labeling the thawed cells after cryopreservation can be limited by the inefficient intracellular labeling and variations in labeling efficiency. Therefore, cryopreservation of the pre-labeled cells can provide uniform cell population and operational convenience for the following in vitro and in vivo investigations. In this study, the feasibility of cryopreserving PSC-derived NPC aggregates labeled with micron-sized particles of iron oxide (MPIO) was investigated. The NPC aggregates derived from embryoid body formation were labeled in suspension with different concentrations of MPIO in the range of 0-100 ug Fe per mL. The results indicated that intracellular MPIO incorporation was retained after cryopreservation (70-80% labeling efficiency), which did not significantly affect cell recovery, proliferation, cytotoxicity and neural lineage commitment. MRI analysis was performed in the phantom tissue environment using cell layers with different MPIO exposures separated by agarose gels. The results showed comparable detectability for the MPIO-labeled cells before and after cryopreservation indicated by T2 and T2* relaxation rates. These findings indicate the feasibility of cryopreserving MPIO-labeled PSC-derived NPC aggregates for potential cell banking toward various in vitro and in vivo cell tracking studies.
Identifier: FSU_migr_etd-9271 (IID)
Submitted Note: A Thesis submitted to the Department of Chemical and Biomedical Engineering in partial fulfillment of the requirements for the degree of Master of Science.
Degree Awarded: Fall Semester, 2014.
Date of Defense: November 5, 2014.
Keywords: Cryopreservation, Iron oxide particle, Magnetic resonnance imaging, Neural progenitor aggregate, Pluripotent stem cell
Bibliography Note: Includes bibliographical references.
Advisory Committee: Yan Li, Professor Directing Thesis; Samuel C. Grant, Committee Member; Jingjiao Guan, Committee Member.
Subject(s): Biomedical engineering
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_migr_etd-9271
Owner Institution: FSU

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Yan, Y. (2014). Cryopreservation of Pluripotent Stem Cell-Derived Neural Progenitor Aggregates Labelled with Micron-Sized Particles of Iron Oxide. Retrieved from http://purl.flvc.org/fsu/fd/FSU_migr_etd-9271