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Comparative Genotype-phenotype Mapping Reveals Distinct Modes of Venom Expression Evolution in the Sympatric Eastern Diamondback Rattlesnake (Crotalus adamanteus) and Eastern Coral SSnake (Micrurus fulvius)

Title: Comparative Genotype-phenotype Mapping Reveals Distinct Modes of Venom Expression Evolution in the Sympatric Eastern Diamondback Rattlesnake (Crotalus adamanteus) and Eastern Coral SSnake (Micrurus fulvius).
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Name(s): Margres, Mark, author
McGivern, James J., author
Seavy, Margaret, author
Wray, Kenneth, author
Facente, Jack, author
Rokyta, Darin, author
Type of Resource: text
Genre: Text
Issuance: serial
Date Issued: 2014
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Selection is predicted to drive diversification within species and lead to local adaptation, but understanding the mechanistic details underlying this process, and thus the genetic basis of adaptive evolution, requires the mapping of genotype to phenotype. Venom is complex and involves many genes, but the specialization of the venom-gland towards toxin production allows specific transcripts to be correlated with specific toxic proteins, establishing a direct link from genotype to phenotype. To determine the extent of expression variation and identify the processes driving patterns of phenotypic diversity, we constructed genotype-phenotype maps and compared range-wide toxin-protein expression variation for two species of snake with nearly identical ranges: the eastern diamondback rattlesnake (Crotalus adamanteus) and the eastern coral snake (Micrurus fulvius). We detected significant expression variation in C. adamanteus, identified the specific loci associated with population differentiation, and found that loci expressed at all levels contributed to this divergence. Contrary to expectations, we found no expression variation in M. fulvius, suggesting that M. fulvius populations are not locally adapted. Our results not only linked expression variation at specific loci to divergence in a polygenic, complex trait, but also have extensive conservation and biomedical implications. Crotalus adamanteus is currently a candidate for Federal listing under the Endangered Species Act, and the loss of any major population would result in the irrevocable loss of a unique venom phenotype. The lack of variation in M. fulvius has significant biomedical application because our data will assist in the development of effective antivenom for this species.
Identifier: FSU_migr_bio_faculty_publications-0001 (IID), 10.1534/genetics.114.172437 (DOI)
Keywords: Genotype-phenotype map, gene expression, adaptation, snake venom
Note: The downloadable document is the authors' original manuscript version (AKA pre-print) of the article which was eventually accepted in the journal Genetics. For the final version of record go to http://www.genetics.org/content/199/1/165'>http://www.genetics.org/content/199/1/165">http://www.genetics.org/content/199/1/165 .
Citation: "Contrasting Modes and Tempos of Venom Expression Evolution in Two Snake Species" Mark J. Margres, James J. McGivern, Margaret Seavy, Kenneth P. Wray, Jack Facente, and Darin R. Rokyta. GENETICS January 2015, 199:165-176 doi:10.1534/genetics.114.172437
Subject(s): Biology
Links: http://dx.doi.org/10.1534/genetics.114.172437
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_migr_bio_faculty_publications-0001
Owner Institution: FSU
Is Part of Series: Department of Biological Science Faculty Publications.
Is Part Of: Genetics.
Issue: 1, 199

Choose the citation style.
Margres, M., McGivern, J. J., Seavy, M., Wray, K., Facente, J., & Rokyta, D. (2014). Comparative Genotype-phenotype Mapping Reveals Distinct Modes of Venom Expression Evolution in the Sympatric Eastern Diamondback Rattlesnake (Crotalus adamanteus) and Eastern Coral SSnake (Micrurus fulvius). Genetics. Retrieved from http://dx.doi.org/10.1534/genetics.114.172437