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Enzymatic Hydrolysis Kinetics

Title: Enzymatic Hydrolysis Kinetics: The Study of the Effects of Glucose on the Conversion of Cellulose to Glucose.
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Name(s): Tacey, Sean, author
Department of Chemical and Biomedical Engineering
Type of Resource: text
Genre: Text
Issuance: serial
Date Issued: 2014
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: (Cellulose, Enzymes, Biofuels) The conversion of cellulose to glucose is one renewable energy strategy under consideration, for glucose can also serve as a feed stock for ethanol and other chemicals currently produced from petroleum. Within sugar production through enzymatic hydrolysis, many steps are believed to occur; this includes the initial adsorption of enzyme onto a cellulose chain, the binding of the enzyme to an accessible site on the chain, the reaction severing the chain, and the motion of the enzyme to another site. Measuring changes that occur during these steps is difficult, but one can separate the adsorption step from the chain removal steps using precise measurements of the mass of well-defined cellulose films. A device that can measure the nanogram amounts of enzyme adsorbed onto a cellulose surface and the change in mass of adsorbed material with time is invaluable in this study; a quartz crystal microbalance (QCM), used in this work, has this potential. Glucose has been found to act as an inhibitor to the overall reaction. Through the QCM, the effects of various glucose concentrations in the enzyme mixture were studied to determine the type of inhibition occurring. While our data seems to show some effects of glucose on the enzymatic process, a statistical analysis of all of the data shows no significant effect on either the enzyme adsorption step or the chain breaking. This gives us reason to believe that the real inhibitory effect of glucose is on the homogeneous reaction that produces glucose from cellobiose.
Identifier: FSU_migr_uhm-0360 (IID)
Keywords: Cellulose, Glucose, Enzymes, Hydrolysis, Quartz Crystal Microbalance, Inhibition
Submitted Note: A Thesis submitted to the Department of Chemical and Biomedical Engineering in partial fulfillment of the requirements for graduation with Honors in the Major.
Degree Awarded: Spring Semester, 2014.
Date of Defense: April 17, 2014.
Subject(s): Chemical engineering
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_migr_uhm-0360
Restrictions on Access: http://creativecommons.org/licenses/by/4.0/
Owner Institution: FSU
Is Part of Series: Honors Theses.

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Tacey, S. (2014). Enzymatic Hydrolysis Kinetics: The Study of the Effects of Glucose on the Conversion of Cellulose to Glucose. Retrieved from http://purl.flvc.org/fsu/fd/FSU_migr_uhm-0360