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Zymosan Fungal Infection Induces Nucleosome Redistributions During the Innate Immune Response

Title: Zymosan Fungal Infection Induces Nucleosome Redistributions During the Innate Immune Response.
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Name(s): Gruder, Olivia, author
Dennis, Jonathan, author
Department of Biological Science
Type of Resource: text
Genre: Text
Issuance: serial
Date Issued: 2014
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Chromatin structure plays a critical role in the regulation of the human genome. An understanding of the role of chromatin structure and its relationship to gene regulation is critical to developing new strategies to prevent and treat diseases. We chose to investigate the anti-inflammatory response of human macrophage like cell line (THP1) to Zymosan, in order to elucidate the regulation of chromatin. Zymosan is a component the fungal cell wall that induces an innate immune response. After THP1 were treated with zymosan, we hypothesized that the fungal infection would initiate an inflammatory response by altering nucleosome redistribution and/or altering chromatin structure in a time dependent manner. Based on previous results that showed rapid, widespread, transient changes in nucleosome distribution in the innate immune response, we chose to look at multiple time points at high temporal resolution: 0 (control), 20', 40', 60', 80', 100', 2h, 3h, 4h and 12h. We measured nucleosome distribution at each of these time points at hundreds of genes transcription start sites involved in the immune response. nucleosome distribution changes in the innate immune response to fungal infection.
Identifier: FSU_migr_uhm-0312 (IID)
Submitted Note: A Thesis submitted to the Department of Biology in partial fulfillment of the requirements for graduation with Honors in the Major.
Degree Awarded: Spring Semester, 2014.
Date of Defense: April 21, 2014.
Subject(s): Biology
Genetics
Genomics
Communicable diseases
Immunology
Medical sciences
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_migr_uhm-0312
Restrictions on Access: http://creativecommons.org/licenses/by/4.0/
Owner Institution: FSU
Is Part of Series: Honors Theses.

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Gruder, O., & Dennis, J. (2014). Zymosan Fungal Infection Induces Nucleosome Redistributions During the Innate Immune Response. Retrieved from http://purl.flvc.org/fsu/fd/FSU_migr_uhm-0312