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Environment-Dependent Single-Molecule Spectroscopy

Title: Environment-Dependent Single-Molecule Spectroscopy.
Name(s): Rodriguez, Anabel, author
Department of Chemistry and Biochemistry
Type of Resource: text
Genre: Text
Issuance: serial
Date Issued: 2013
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: The dependence of frame rate on fluorescence correlation studies of "on" events in CdSe nanoparticles is investigated. Average Pearson's correlation coefficient (r) values decrease at a greater rate for particles imaged at a frame rate below 70 s-1 than at frame rates between 70 s-1 and 200 s-1. To better understand this trend, the same particle is imaged for all frame rates and data shows high variability in r values. Overall, data suggests that (1) application of correlation analysis on future studies pertaining to the influence of environment on nanoparticle fluorescence will need to be done on the same particle and (2) r values may only be comparable for results obtained at the same frame rate for the same particle. The data suggests that r values themselves may not be useful to analyze correlations between on events but a change in the r value for the same particle imaged at a certain frame rate under different environmental conditions may give some insight into environmental influences on fluorescence.
Identifier: FSU_migr_uhm-0248 (IID)
Keywords: Single-Molecule Spectroscopy, Fluorescence Intermittency, Pearson's Correlation Coefficient Analysis
Submitted Note: A Thesis submitted to the Department of Chemistry and Biochemistry in partial fulfillment of the requirements for graduation with Honors in the Major.
Degree Awarded: Summer Semester, 2013.
Date of Defense: August 2, 2013.
Subject(s): Chemistry, Physical and theoretical
Persistent Link to This Record:
Restrictions on Access:
Owner Institution: FSU
Is Part of Series: Honors Theses.

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Rodriguez, A. (2013). Environment-Dependent Single-Molecule Spectroscopy. Retrieved from