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Study of Glucose as an Inhibitor to the Enzymatic Hydrolysis of Cellulose Using a Quartz Crystal Microbalance

Title: A Study of Glucose as an Inhibitor to the Enzymatic Hydrolysis of Cellulose Using a Quartz Crystal Microbalance.
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Name(s): Quintanilla, Adam, author
Department of Chemical and Biomedical Engineering
Type of Resource: text
Genre: Text
Issuance: serial
Date Issued: 2013
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Cellulose derived from lignocellulose has become the focus of many groups globally as a potential replacement for biofuel feedstock production. The most important step involved in this process is the enzymatic hydrolysis of cellulose. The Quartz Crystal Microbalance (QCM) has recently shown value in its ability to analyze hydrolysis in real time while controlling important reaction variables. The two main points of interest are the mass of enzyme bound to cellulose and the rate at which it is removed by enzymes. Hydrolysis was run at three enzyme concentrations: 0.0613 FPU/mL, 0.1247 FPU/mL, and 0.2475 FPU/mL. It has been also shown in multiple studies that glucose could be acting as an inhibitor to cellulase. Four glucose concentrations were observed at each enzyme concentration: 1 mg/mL, 2 mg/mL, 5 mg/mL, and 10 mg/mL. Analysis of the data revealed that there was a significant relationship between enzyme concentration and both the mass of enzyme bound and the rate of removal. The effects were determined to not be linear in nature, but more likely follow a model which suggests a maximum possible binding and rate. The effect of glucose on enzyme binding was insignificant due to large standard deviations seen amongst all conditions. This insignificance was strengthened by a two-way analysis of variance which revealed that the data collected was not repeatable. The overall effect of glucose on hydrolysis rates was negative in that rates generally decreased. The type of inhibition was determined to be competitive using an inverse rate plot versus glucose concentration.
Identifier: FSU_migr_uhm-0179 (IID)
Keywords: Hydrolysis, QCM, Cellulose
Submitted Note: A Thesis submitted to the Department of Chemical & Biomedical Engineering in partial fulfillment of the requirements for graduation with Honors in the Major.
Degree Awarded: Spring Semester, 2013.
Date of Defense: April 19, 2013.
Subject(s): Chemical engineering
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_migr_uhm-0179
Owner Institution: FSU
Is Part of Series: Honors Theses.

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Quintanilla, A. (2013). A Study of Glucose as an Inhibitor to the Enzymatic Hydrolysis of Cellulose Using a Quartz Crystal Microbalance. Retrieved from http://purl.flvc.org/fsu/fd/FSU_migr_uhm-0179