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Characterization of the Role of Eco1 in Chromosome Segregation During Meiosis in Budding Yeast

Title: Characterization of the Role of Eco1 in Chromosome Segregation During Meiosis in Budding Yeast.
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Name(s): Reynolds, Torrie, author
Department of Biological Science
Type of Resource: text
Genre: Text
Issuance: serial
Date Issued: 2012
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: To ensure accurate chromosome segregation, sister chromatid cohesion must be properly established at S-phase when DNA is replicated. The protein Eco1/Ctf7 is responsible for establishing this cohesion, and is the focus of this Thesis work. The conserved protein complex cohesin acts as the "molecular glue" that mediates this sister chromatid cohesion. Cohesin is loaded onto the chromosomes before DNA replication, and when the acetyltransferase Eco1 acetylates cohesin at S-phase, the sister chromatids become entrapped in the cohesin ring. This Thesis aimed to elucidate the roles of Eco1 in cohesin-mediated sister chromatid cohesion, specifically during meiosis. A novel eco1 meiosis-specific mutant was constructed in the budding yeast Saccharomyces cerevisiae. Fluorescence microscopy techniques were used to assay sister chromatid cohesion, nuclear division, and chromosome structure in cells depleted of Eco1 in meiosis. This work shows that meiotic Eco1 and its establishment of sister chromatid cohesion regulates recombination and chromosome segregation during meiosis.
Identifier: FSU_migr_uhm-0137 (IID)
Keywords: Meiosis, Cohesion, Eco1
Submitted Note: A Thesis submitted to the Department of Biological Science in partial fulfillment of the requirements for the degree of Honors in the Major.
Degree Awarded: Fall Semester, 2012.
Date of Defense: November 6, 2012.
Subject(s): Cytology
Molecular genetics
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_migr_uhm-0137
Owner Institution: FSU
Is Part of Series: Honors Theses.

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Reynolds, T. (2012). Characterization of the Role of Eco1 in Chromosome Segregation During Meiosis in Budding Yeast. Retrieved from http://purl.flvc.org/fsu/fd/FSU_migr_uhm-0137